中国生态农业学报2017，Vol.25Issue(6)：893-902,10.DOI:10.13930/j.cnki.cjea.161092
黑豆种质苗期耐荫性评价及其根系对弱光胁迫的响应
Evaluation for shade tolerance of black soybean germplasms and their root structure response to shade stress at seedling stage
摘要
Abstract
Maize-soybean relay strip intercropping systems is an important planting mode in Southwest China. However,shade-tolerant germplasms of black soybean are scarce and the mechanism of shade tolerance of black soybean is still unclear, which significantly affects further promotion of the mode. The objective of this study was to establish a mathematical model to evaluate shade-tolerance ability of black soybean seedlings, screen shade-tolerance appraisal indicators and discuss the diverse responses of the root structures of different black soybean germplasms seedlings to shade stress.The study was also intended to lay the foundation for breeding varieties and for explaining the mechanism of shade tolerance of black soybean. Thus in the study, shade tolerance of 23 black soybean germplasms were evaluated in a pot culture experiment with normal light and shade treatments. The morphological characteristics (plant height, stem diameter, hypocotyl length, leaf dry weight, stem dry weight, main stem count, total root length, root surface area, total root volume, etc.) and physiological parameters were measured at the 3rd trifoliolate leaf stage (V3). An evaluation mathematical model was established using multivariate statistical analysis. Additionally, based on the evaluation results, correlation analysis was conducted to determine differential responses of roots to shade stress. The 23 black soybean germplasms were divided into three groups, including tolerant, moderately tolerant and sensitive germplasms. A total of 5 evaluation indexes closely related to shade tolerance were screened out. The study showed that the response of root structure to shade stress varied with shade-tolerance ability of black soybean germplasms. Root pa-rameters such as total root length, root surface area, total root volume and root dry weight all had a similar order, which was shade tolerant germplasms > moderate shade tolerant germplasms > sensitive germplasms. The results indicated that shade tolerant germplasms of black soybean had a stronger root system.关键词
黑豆/苗期/耐荫性/多元统计分析/根系结构Key words
Black soybean/Seedling stage/Shade tolerance/Multivariable statistics/Root structure分类
农业科技引用本文复制引用
杨才琼,胡宝予,吴海军,秦雯婷,张潇文,刘卫国,杨文钰,刘江..黑豆种质苗期耐荫性评价及其根系对弱光胁迫的响应[J].中国生态农业学报,2017,25(6):893-902,10.基金项目
国家自然科学基金项目(31301277,31401329)和中国博士后科学基金面上项目(2014M560724)资助 This study was supported by the National Natural Science Foundation of China (31301277, 31401329) and the China Postdoctoral Science Foundation (2014M560724). （31301277,31401329）
