生物技术通报2017，Vol.33Issue(6)：89-96,8.DOI:10.13560/j.cnki.biotech.bull.1985.2016-1163
桂花类胡萝卜素异构酶基因的克隆及表达分析
Cloning and Expression Analysis of Carotenoid Isomerase Gene in Osmanthus fragrans
摘要
Abstract
Based on the sequence information of Unigene of Osmanthus fragrans'Yanhong Gui'from the petal transcriptome database, sequence of open reading frame in one carotenoid isomerase gene(OfCRTISO)from Osmanthus fragrans was amplified using RT-PCR technology and confirmed by sequencing. Bioinformatics analysis indicated that OfCRTISO contained a 1842 bp open reading frame,encoding 613 amino acid residues. OfCRTISO from Osmanthus fragrans shared high sequence similarity with CRTISOs from Pedaliaceae and Solanaceae plants. Phylogenetic analysis showed that OfCRTISO and Sesamum indicum CRTISO(XP_011077785)gathered into one branch,revealing that these two proteins showed the closest genetic relationship. Gene expression analysis implicated that the expression level of OfCRTISO was extremely low at bud former stage,gradually increased at bud later stage,and reached the peak at initial flowering stage ;however,it significantly decreased at full flowering stage. According to the expression analysis of OfCRTISO in the petals of sweet osmanthus cultivars with different flower color,it was demonstrated that the expression level of OfCRTISO at initial flowering stage might have an impact on the carotenoid accumulation in the petals of sweet osmanthus cultivars at full flowering stage.关键词
桂花/花色/类胡萝卜素/类胡萝卜素异构酶基因Key words
Osmanthus fragransl/petal color/carotenoids/carotenoid isomerase gene引用本文复制引用
张超,刘玉成,王艺光,付建新,赵宏波..桂花类胡萝卜素异构酶基因的克隆及表达分析[J].生物技术通报,2017,33(6):89-96,8.基金项目
国家自然科学基金项目(31501790),浙江省自然科学基金(LQ15C160004 和 LQ16C160003),浙江农林大学科研发展基金人才启动项目(2016FR031) （31501790）
