生物技术通报2017，Vol.33Issue(6)：112-120,9.DOI:10.13560/j.cnki.biotech.bull.1985.2016-1216
四种芽孢杆菌对蒜薹灰葡萄孢霉抑制作用的时效性研究
Timeliness of Four Bacillus Strains Against Botrytis cinerea of Garlic Sprouts
摘要
Abstract
The aim of this work is to identify the four strains of Bacillus at the species level and study their inhibitory effects on Botrytis cinerea in vitro and their timeliness of fresh-keeping garlic sprouts in vivo. The plate-to-plate method and the mycelial growth method were used for in vitro experiments,and the injury inoculation method was for prevention and therapy treatment in vivo experiments in different periods. Results showed that four Bacillus strains in vitro presented significant inhibitory effects on colonization and mycelial growth of Botrytis cinerea in contrast to the control,and in the order of HB-2 > B579 > B1> B001(P < 0.05). In vivo experiments under 20℃ showed that preventive treatments with four Bacillus strains effectively controlled the expansion of B. cinerea lesion,and the effect was better than that of therapy treatment,but the optimal preventive time of various Bacillus varied. Among them,the preventive treatment with Bacillus HB-2 and B579 better maintained the soluble solids,vitamin C and titratable acid content of garlic sprouts. In conclusion,Bacillus HB-2 is more effective than the other three strains of Bacillus to prevent garlic mold of garlic sprouts.关键词
芽孢杆菌/灰葡萄孢霉/蒜薹保鲜/时效性Key words
Bacillus/Botrytis cinerea/garlic sprouts fresh-keeping/timeliness引用本文复制引用
魏倩,张娜,张平,李明刚,阎瑞香..四种芽孢杆菌对蒜薹灰葡萄孢霉抑制作用的时效性研究[J].生物技术通报,2017,33(6):112-120,9.基金项目
国家科技支撑项目(2015BAD16B07),河南省科技开发合作计划项目(152106000060) （2015BAD16B07）
