生物技术通报2017，Vol.33Issue(6)：121-127,7.DOI:10.13560/j.cnki.biotech.bull.1985.2016-1121
内生链霉菌SSD49的抑菌活性和防病促生效果
Antimicrobial Activities and Efficacy of Endophytic Streptomyces sp. SSD49 in Plant Disease Control and Plant-growth-promoting
摘要
Abstract
The endophytic Streptomyces sp. SSD49,isolated from the roots of Strophanthus divaricatus,showed strong antifungal activity against Botryosphaeria dothidea. In order to analyze its antimicrobial activity against various pathogens and the characterization of plant-growth-promoting as well as the potential of biocontrol agents,a series of experiments were conducted,including plat confrontation,tissue culture and potting test in greenhouse. The results showed that Streptomyces sp. SSD49 inhibited the bacterial pathogens of Cryphonectria parasitica, Sclerotinia sclerotiorum,Phytophthora capsici,Dothiorella gregaria,Lonsdalea quercina subsp. populi,as well as plant pathogens and human opportunistic pathogens of Methicillin-resistant Staphylococcus aureus and Candida albicans. It secreted chitinase and produced indole acetic acid at the concentration of 31.56 mg/L. The shoot height,root length,shoot and root dry weight of Populus tomentosa tissue seedlings,soybean,tomato and pepper plants inoculated with strain SSD49 increased by 16.98-41.82%,19.00-55.85%,4.58-70.87% and 15.25-126.06%,respectively. The inhibition rate to Sclerotinia sclerotiorum reached 88.24%. The above results demonstrated that endophytic Streptomyces sp. SSD49 is potentially applicable in controlling plant disease in agriculture and forestry,while the mechanism of plant disease control and plant-growth-promoting needs to be further studied.关键词
植物内生菌/内生链霉菌/抑菌活性/生物防治/植物促生Key words
endophyte/endophytic Streptomyces/antimicrobial activities/plant disease control/plant-growth-promoting引用本文复制引用
葛优优,刘晓瑜,窦桂铭,马玉超..内生链霉菌SSD49的抑菌活性和防病促生效果[J].生物技术通报,2017,33(6):121-127,7.基金项目
国家林业局公益项目(201304409),北京市科技新星项目(2011033),国家自然科学基金项目(J1103516) （201304409）
