生物技术通报2017，Vol.33Issue(6)：162-169,8.DOI:10.13560/j.cnki.biotech.bull.1985.2016-1020
酵母抽提物对CHO细胞生长及抗体表达的影响
Effects of Yeast Extract on Cell Growth and Antibody Production in CHO Cell Culture
摘要
Abstract
The objective is to get insights into the effects of yeast extract on Chinese hamster ovary(CHO)cell growth and monoclonal antibody production. We comprehensively investigated the cell growth,antibody production and nutrients metabolism at different yeast extract concentrations in continuous passage culture and batch culture. It was found that yeast extract significantly improved CHO cell growth at low concentration(1 g/L)and remarkably inhibited CHO cell growth at high concentration(5-10 g/L)during continuous passage culture. Additionally,the result of seed cells in batch culture was not influenced while adding the yeast extract during continuous passage culture. Supplementing yeast extract in batch culture medium,a positive correlation was found between specific antibody production rate and the yeast extract concentration with a maximal antibody titer achieved at yeast extract concentrations of 10 g/L. In conclusion,yeast extracts can make a great contribution on animal cell culture process with a strategy of low concentration added at cell growth phase and high concentration added at protein-production phase.关键词
动物细胞培养/CHO细胞/酵母抽提物/单克隆抗体/氨基酸代谢Key words
animal cell culture/CHO cells/yeast extract/monoclonal antibody/amino acid metabolism引用本文复制引用
胡冬冬,赵亮,范里,刘旭平,邓献存,缪仕伟,谭文松..酵母抽提物对CHO细胞生长及抗体表达的影响[J].生物技术通报,2017,33(6):162-169,8.基金项目
国家自然科学基金项目(21406066),国家重大专项(2013ZX10004003-003-003) （21406066）
