山西农业大学学报（自然科学版）2017，Vol.37Issue(4)：263-267,5.
牛卵泡可卡因-苯丙胺调节转录肽候选受体的筛选
Bovine follicular conine-and amphetamine-regulate transcript candidate receptors screening
摘要
Abstract
[Objective]This study was performed to screen the candidate receptors of Cocnine-and Amphetamine-Regulate Transcript(CART)of bovine follicles.[Methods]35325 expressed genes obtained from bovine follicular transcriptome after depth of sequencing were screened in this study,selecting out some genes that ODF1/ODF2 tending to infinity and the ratio between 1.5 to 2.0 to identify their gene symbol.Then NCBI and CELLULAR were used to identify subcellular localization,transmembrane helix software was also used in the prediction of transmembrane α helix region,screening out 7 αt helix composed of transmembrane region of membrane protein and CART candidate receptor.[Results]Eventually,the 11 highly expressed genes were screened by differential gene expression,subcellular localization and transmembrane helix prediction.According to the structure and function of the screened 23 G protein-coupled receptor and neuropeptide receptor binding characteristics,we find four G-protein-coupled receptors as CART candidate receptors.[Conclusion]The AGTR2、HTR2B、S1PR1 and S1PR2 as CART candidate receptors ultimately,and laid a solid foundation for further study.关键词
牛卵泡/CART/候选受体/跨膜螺旋/表达基因/G蛋白偶联受体Key words
Bovine follicle/CART/Candidate receptor/Transmembrane helical/Expressed genes/G protein coupled-receptor分类
农业科技引用本文复制引用
王锴,孟金柱,李鹏飞,毕锡麟,景炅婕,张子宁,吕丽华..牛卵泡可卡因-苯丙胺调节转录肽候选受体的筛选[J].山西农业大学学报（自然科学版）,2017,37(4):263-267,5.基金项目
国家自然基金(31172211) （31172211）
山西省回国留学人员科研资助项目(2014-重点5) （2014-重点5）
山西省国际科技合作项目(201603D421006) （201603D421006）
山西省三晋学者和人才引进项目 （）
山西农业大学引进人才博士科研启动费(2014ZZ04) （2014ZZ04）
山西农业大学创新金项目(zdpy201403/201503) （zdpy201403/201503）
