山西农业大学学报（自然科学版）2017，Vol.37Issue(4)：287-294,8.
小球藻无菌培养体系的建立及培养条件的优化
The establishment of sterile culture system and optimization of culture conditions from Erichsen chlorella
摘要
Abstract
[Objective]Erichsen Chlorella is a promising candidate for biofuel production for its high lipid content and photosynthetic efficiency.However,contamination and cultivation conditions controlling are two important factors affecting the microalgal biomass and lipid accumulation,as well as the large scale production of Chlorella.[Method]In this study,5 antibiotics cefazolin and Cephalosporins(Cefo),Ampicillin(Amp),Carbenicillin(Car),Neomycin sulfateon(Nw) and rifampicin and amphotericin (Rif) were tested as bacteriostat with different concentrations and combinations,the effect of the antibiotics on the bacteria inhibition and microalgal growth were explored.In addition,the effects of inoculum,medium culture pH,cultivation temperature and salt concentration in culture medium on Chlorella growth were also studied.[Results]As a result,a sterile system for Chlorella cultivation was established and the cultivation conditions were optimized then.[Conclusion]Results showed that the combination of 30 mL· L-1 Rif and 100 mL·L-1 Cefo was effectively bacteriostatic in the cultivation system of Chlorella.And the optimal conditions for Chlorella growth were as follows:OD 0.2 for initial inoculum,p H range 8.5-9.5,cultivation temperature(25 ± 0.5) ℃ and salt density lower than 0.01 mol· L-1.关键词
埃氏小球藻/抗生素/无菌培养体系/培养条件优化Key words
Erichsen Chlorella/Antibiotics/Sterile system/Optimization of cultivation conditions分类
生物科学引用本文复制引用
武振晋,周广航,赵奎,王亚君,季春丽,薛金爱,李润植..小球藻无菌培养体系的建立及培养条件的优化[J].山西农业大学学报（自然科学版）,2017,37(4):287-294,8.基金项目
国家“948”项目(2014-Z39) （2014-Z39）
山西省煤基重点科技攻关项目(FT-2014-01) （FT-2014-01）
