生殖医学杂志2017，Vol.26Issue(6)：585-591,7.DOI:10.3969/j.issn.1004-3845.2017.06.015
卵丘细胞上AIFM2和FDX1的表达及其与小鼠卵母细胞成熟的关系
Expressions of apoptosis-inducing factor mitochondrion-associated 2 and ferredoxin 1 in mouse cumulus cells derived from in vitro or in vivo maturated follicles
摘要
Abstract
Objective: To detect the expressions of apoptosis-inducing factor mitochondrion-associated 2 (AIFM2) and ferredoxin 1 (FDX1) mRNA in the cumulus cells (CCs) around the cumulus oocyte complex (COCs) undergoing in vitro maturation (IVM) or in vivo maturation,and investigate their potential functions in regulating oocyte maturation. Methods: In vivo maturation COCs (IVV-COCs) and germinal vesicle COCs (GV-COCs) were isolated from 3 weeks old mice,and then GV-COCs were cultured to metaphase Ⅱ stage (IVM-COCs) by in vitro maturation. The CCs were collected from GV-COCs,IVM-COCs and IVV-COCs respectively,and defined as GV-CCs group,IVM-CCs group and IVV-CCs group. The expression of AIFM2 and FDX1 mRNA were detected by RT-qRCR. The sub-cellular location of FDX1 protein and AIFM2 protein in GV-COCs,IVM-COCs and IVV-COCs were observed by laser scanning confocal microscope. Results: The expression of AIFM2 mRNA in IVM-CCs group [(0.72±0.01)] and IVV-CCs group [(0.67±0.02)] was significantly higher than that in GV-CCs group [(0.10±0.06)] (P<0.01). The expression of FDX1 mRNA in IVM-CCs group [(1.58±0.35)] and IVV-CCs group [(1.82±0.34)] was also significantly higher than that in GV-CCs group [(0.10±0.03)] (P<0.01). However,there was no significantly difference in the expression of AIFM2 and FDX1 between IVV-CCs group and IVM-CCs group (P>0.05). The immunofluorescence results showed that both AIFM2 and FDX1 were mainly located in the cytoplasm of CCs and expressed in each layer of GV-COCs,which were also expressed in oocytes at GV stage. The number of positive cells in IVM-COCs and IVV-COCs increased compared with GV. Conclusions: After oocyte maturation,the expression of AIFM2 and FDX1 is increased in CCs,indicating that AIFM2 and FDX1 are involved in the process of oocyte maturation. It is expected to be a biomarker for predicting oocyte maturation.关键词
卵丘细胞/AIFM2/FDX1/卵丘-卵母细胞复合体/线粒体代谢Key words
Cumulus cells/Apoptosis-inducing factor mitochondrion-associated 2/Ferredoxin 1/Cumulus oocyte complex/Mitochondria metabolism引用本文复制引用
周团萍,千日成,邵丽,徐祎欣,孟艳,刘凯鲁,高超,高莉,刘嘉茵,崔毓桂..卵丘细胞上AIFM2和FDX1的表达及其与小鼠卵母细胞成熟的关系[J].生殖医学杂志,2017,26(6):585-591,7.基金项目
国家自然科学基金(81270746,81200439) （81270746,81200439）
卫生部行业科研专项课题(201402004,201302013,201002013) （201402004,201302013,201002013）
