生殖医学杂志2017，Vol.26Issue(6)：592-598,7.DOI:10.3969/j.issn.1004-3845.2017.06.016
用模拟经期内膜组织建立子宫内膜异位症小鼠模型及其生殖能力的研究
Establishment of mouse endometriosis model by injecting simulated menstrual endometrial tissue and its reproductive ability
摘要
Abstract
Objective: To establish mouse model of endometriosis (EM) to observe its fertility,and explore the possible factors affecting the reproductive ability. Methods: Fifty one female mice aged 8 weeks were used as recipient mice and randomly divided into model group (n=21),sham operation group (n=15) and blank group (n=15). The model mice were established by intraperitoneal injection of simulating menstrual endometrial tissue of donor mice. The mice of sham group were established by injecting fragments of abdominal adipose tissue from donor mice into peritoneal cavity. The mice of blank group were not injected intraperitoneally. The three groups of mice were observed in estrus cycle,and the blood samples were collected at diestrus. The mice of three groups were mated with male mice to appear vaginal plug. The number of embryos,pregnancy rate,absorptive fetus rate and so on were counted in each group of mice. The serum levels of FSH,LH,carbohydrate antigen 125 (CA125) and interleukin-2 (IL-2) in the peritoneal fluid were measured by enzyme-linked immunosorbent assay (ELISA) and compared among the groups. Results: The mouse model of EM was successfully established. The estrous cycle of model group was normal. The pregnancy rate of model group is significantly lower than the sham group and blank group (P<0.05). However there were no significant differences in absorptive fetus rate,teratogenic rate,pup weight,and crown-rump length among the three groups (P>0.05). The serum FSH levels and IL-2 concentration of infertile mice in model group were significantly higher than the three groups before mating (P<0.01). There were no statistical differences in the levels of serum LH and CA125 among the groups (P>0.05). Conclusions: The mouse endometriosis model is successfully established. In the model mice,the ovarian function and the peritoneal immune microenvironment were affected in infertile mice,which may be the important reason for the decrease of the reproductive ability.关键词
子宫内膜异位症/小鼠模型/生殖能力Key words
Endometriosis/Mouse model/Reproductive ability引用本文复制引用
王汝倩,张洁,刘璐茜,翟东霞,张丹英,姚睿嫔,吉翔,程雯,俞超芹..用模拟经期内膜组织建立子宫内膜异位症小鼠模型及其生殖能力的研究[J].生殖医学杂志,2017,26(6):592-598,7.基金项目
国家自然科学基金(81573755,81503604) （81573755,81503604）
上海市中医药三年行动计划(ZY3-CCCX-3-7002) （ZY3-CCCX-3-7002）
