生殖医学杂志2017，Vol.26Issue(6)：599-604,6.DOI:10.3969/j.issn.1004-3845.2017.06.017
孕酮回植激活Akt/GSK3β信号通路逆转月经发生
Implanted progesterone reverses menstruation by activating Akt/GSK3 beta signaling pathway
摘要
Abstract
Objective: To investigate the effects of implanted progesterone at the critical period before menstrual occurrence induced by progesterone withdrawal on the Akt/GSK3 beta signaling pathway in mice menstrual-like model. Methods: Thirty ovariectomized C57BL/6J female mice with menstrual-like model were randomly divided into three groups: 12 hours after progesterone withdrawal group (12 hours group),16 hours after progesterone withdrawal group (16 hours group),12 hours after progesterone withdrawal followed by implantation of progesterone for 4 hours group (implanted progesterone group),ten mice were for each group. The uterus horns of mouse were collected at 12 hours & 16 hours after progesterone withdrawal,and 12 hours after progesterone withdrawal followed by implantation of progesterone for 4 hours. The changes of Akt,p-Akt,GSK3β and p-GSK3β expression in mouse uterine tissue were detected by immunohistochemistry,Western blotting and qRT-PCR respectively. Results: The expression of Akt mRNA of mouse uterine tissue in 12 hours group was significantly higher than implanted progesterone group and 16 hours group (P<0.05),and there was no significantly different between the implanted progesterone group and 16 hours group (P>0.05). The expression of GSK3β mRNA in the 12 hours group was significantly higher than that in the implanted progesterone group and 16 hours group (P<0.05). The expression of p-Akt and p-GSK3β in endometrial tissue in 16 hours group was significantly lower than that in 12 hours group and implanted progesterone group (P<0.05). Immunohistochemical results showed that the expression of p-Akt and p-GSK3β protein presented similar distribution patterns in mouse uterine tissues,and p-Akt and p-GSK3β protein were widely distributed in stromal cells,glandular epithelium and luminal epithelial cells in decidual areas. Conclusions: Implanted progesterone at the critical period,12 hours after progesterone withdrawal,reverses menstruation by rapidly activating Akt/GSK3 beta signaling pathway and its downstream cascade reaction through non-transcriptional mechanisms.关键词
蛋白激酶B/糖原合成激酶3β/孕酮撤退/月经逆转Key words
Akt/GSK3 beta/Progesterone withdrawal/Reversal of Menstruation引用本文复制引用
时灿灿,于明明,张先平,陈丽刚,王乾兴..孕酮回植激活Akt/GSK3β信号通路逆转月经发生[J].生殖医学杂志,2017,26(6):599-604,6.基金项目
贵州省科技厅联合基金项目(黔科合J字LKZ[2010]33号) （黔科合J字LKZ[2010]33号）
贵州省科学技术基金资助项目(黔科合J字[2011]2282) （黔科合J字[2011]2282）
