天然产物研究与开发2017，Vol.29Issue(4)：553-558,6.DOI:10.16333/j.1001-6880.2017.4.003
油茶蒲提取物抑制前列腺增生活性化合物的分离与鉴定
Isolation and Identification of Active Compounds on Benign Prostate Hyperplasia from Oiltea camellia Extract
摘要
Abstract
In order to investigate bioactive constituents with inhibiting effect on benign prostatic hyperplasia from Oiltea camellia,preparative high-performance liquid chromatography (preparative HPLC) was adopted to isolate key bioactive compounds from polyamide fraction of O.camellia extracts(OCE).The chemical structures of those compounds were identified using mass spectrometer(MS) and nuclear magnetic resonance(NMR) analyses.In addition,its growth inhibitory activity against BPH-1 cells was detected by MTT assay.The results indicated that F1,F2 and F3,as main active substances in Fr8,showed stronger inhibitory effect on BPH-1 cells at low doses (50 μg/mL) than that of positive control finasteride(26.50％),with the inhibition rates of 30.31％,43.26％ and 29.00％,respectively.F1,F2 and F3 were identified as valoneic acid dilactone,3-O-galloyl-4,6-[(S)-hexahydroxydiphenoyl]-(α/β)-D-glucopyranose and 3,4,6-tri-O-galloyl-(α/β)-D-glucose,respectively.This study provided theoretical reference and material foundation for the development of natural products on the improvement of prostate disease.关键词
油茶蒲/聚酰胺组分/制备液相/结构鉴定/人前列腺增生细胞Key words
Oiltea camellia/polyamide fraction/preparative high-performance liquid chromatography/structural identification/BPH-1 cell分类
医药卫生引用本文复制引用
周琛媛,董柳青,赵波,吴晓琴,张英,沈建福..油茶蒲提取物抑制前列腺增生活性化合物的分离与鉴定[J].天然产物研究与开发,2017,29(4):553-558,6.基金项目
国家自然科学基金面上项目(31270722) （31270722）
