天然产物研究与开发2017，Vol.29Issue(4)：563-568,6.DOI:10.16333/j.1001-6880.2017.4.005
异槲皮苷对叔丁基过氧化氢诱导的L-02细胞氧化损伤的保护作用
Protective Effect of Isoquercitrin on t-BHP-induced Oxidative Damage of L-02 Cells
摘要
Abstract
To investigate the protective effect of isoquercitrin on t-BHP-induced oxidative damage of L-02 cells.In vitro oxidative stress damage model was established by treating L-02 cells with t-BHP(100 μmol/L) for 24 h.Cells were divided into control case,model case (100 μmol/L t-BHP),positive control case (10 μmol/L Vitamin E,VE) and isoquercitrin(1,10 and 100 μmol/L) cases.Cell viability was determined by MTT assay.Contents of LDH,ROS,MDA,GSH-Px and SOD activities were detected by ELISA methods.The protein expressions of sirtuin 6 (Sirt6) and nuclear factor-k-gene binding p65 (NF-κBp65) were determined by Western blot technology.Compared with model case,isoquercitrin significantly enhanced cell viability,GSH-Px and SOD activities,and decreased LDH activity,contents of ROS and MDA.Western blot showed isoquercitrin upregulated the expression of Sirt6 and downregulated the expression of NFκBp65.Isoquercitrin showed the protective effect on t-BHP-induced oxidative damage of L-02 cells and its protective effects may be related with increasing in ROS removal ability and regulation of Sirt6 and NF-κBp65 level.关键词
异槲皮苷/L-02细胞/氧化损伤Key words
isoquercitrin/L-02 cells/oxidative damage分类
医药卫生引用本文复制引用
雷鸣,徐应淑,孙静波,刘双,龚其海,高健美..异槲皮苷对叔丁基过氧化氢诱导的L-02细胞氧化损伤的保护作用[J].天然产物研究与开发,2017,29(4):563-568,6.基金项目
贵州省中医药、民族医药科学技术研究专项(QZyy-2014-029) （QZyy-2014-029）
贵州省科技创新人才团队[2015-4023] （）
贵州省高层次创新人才“百”层次(20165684) （20165684）
