天然产物研究与开发2017，Vol.29Issue(4)：569-574,6.DOI:10.16333/j.1001-6880.2017.4.006
霍山石斛水提取物通过介导NF-κB/p65和p38MAPK减轻小鼠酒精性肝损伤
Protective Effect of Dendrobium huoshanense C.Z.Tang et S.J.Cheng Water Extract on Alcohol-induced Acute Liver Injury by Inhibiting NF-κB/p65 and p38 MAPK
摘要
Abstract
Alcoholic hepatic injury is caused by the toxicity of long-term drinking attracts more and more attention in recent years.Increasing studies have shown that a variety of bioactive ingredients in Dendrobium huoshanense C.Z.Tang et S.J.Cheng(DHC) possessed protective effect on alcoholic liver damage.In present study,the protective effect of DHC on alcoholic liver damage was evaluated and the possible underlying mechanism was explored.This study aimed to provide a reliable theoretical basis for the domain of protecting liver for DHC.The hepatoprotective effects of DHC in a mouse model of acute alcohol-induced liver injury were evaluated based on biochemical indicators and antioxidative capacity of serum and liver in 40 d.Severe liver damage caused by 52％ ethanol intake with increasing activation of hepatic markers was decreased in the group of mice fed DHC,and the results were confirmed through hematoxylin and eosin staining.Serum biochemical indicators and antioxidative capacity were abnormal by intragastric administration of 52％ ethanol,but recovered by DHC.Fttrthermore,DHC increased the activity of antioxidant enzymes and reduced inflammatory reactions in liver.The experimental results indicate that DHC had prevcntive and therapeutic effects against acute alcohol-induced liver injury because it could get down the levels of ALT,AST,CRP,TNFα,MDA,and make up the levels of SOD.DHC treatment significantly decreased the relative levels of NF-κB/p65 and the phosphorylation of p38 MAPK.Therefore,the liver effects of DHC may be attributed to its antioxidant and anti-in? ammatory activities,NF-κB/p65 and p38 MAPK may be play important roles in response to acute liver injury and are involved in the inflammatory response and oxidative injury关键词
霍山石斛/酒精性肝损伤/抗氧化/抗炎/NF-κB/p65/p38 MAPKKey words
Dendrobium huoshanense C.Z.Tang et S.J.Cheng water extracts/alcoholic hepatic injury/antioxidant/anti-in? ammatory/NF-κB/p65/p38 MAPK分类
医药卫生引用本文复制引用
王凤华,韩吉春,李德芳,房雪,郑秋生..霍山石斛水提取物通过介导NF-κB/p65和p38MAPK减轻小鼠酒精性肝损伤[J].天然产物研究与开发,2017,29(4):569-574,6.基金项目
滨州医学院科研启动基金(BY2014KYQD01) （BY2014KYQD01）
山东省高等学校优势学科人才团队培育计划(LJRZ201605 2410,李德芳) （LJRZ201605 2410,李德芳）
