天然产物研究与开发2017，Vol.29Issue(4)：605-609,5.DOI:10.16333/j.1001-6880.2017.4.013
超高液相色谱法同时测定北五味子中7种木脂素类化合物
Simultaneous Determination of Seven Lignans from Schisandrae Chinensis Fructus by Ultra-performance Liquid Chromatography
摘要
Abstract
In this study,an ultra-performance liquid chromatography (UPLC) was developed for the simultaneous determination of 7 lignans from Schisandrachinensis fruits.The analytes were separated on an ACQUITY UPLC(R) BEH C18 column(50 mm ×2.1 mm,1.7 μm) with acetonitrile-water as mobile phases in gradient elution under 254 nm.The flow rate was 0.4 mL/min and column temperature was set as 30 ℃.The injection volume was 2 μL.By using the optimized method,the detection limits of the 7 lignans ranged from0.0105 to 0.1600 μg/mL,the limits of quantitation ranged from 0.1050 to 0.8200 μg/mL.The recoveries of the 7 lignans in the samples were 95.09％ to 104.5％ with relative standard deviation(RSD％) ranged from 0.0340％ to 1.3800％.The established UPLC method for the simultaneous determination of 7 lignans can provide a more comprehensive reference for the quality control of S.chinensi.关键词
北五味子/超高效液相色谱法/木脂素类化合物/同时测定Key words
Schisandra chinensis/ultra-high performance liquid chromatography/lignans/simultaneous determination分类
医药卫生引用本文复制引用
白宏博,冯志强,史鑫波,孙晓春,刘妍如,唐志书..超高液相色谱法同时测定北五味子中7种木脂素类化合物[J].天然产物研究与开发,2017,29(4):605-609,5.基金项目
国家自然科学基金(81501229) （81501229）
陕西省协同创新计划(2015XT-35) （2015XT-35）
陕西省教育厅专项科研计划(320104-203010012) （320104-203010012）
陕西省科技统筹创新工程计划(2011KTCL03-05) （2011KTCL03-05）
