天然产物研究与开发2017，Vol.29Issue(4)：671-679,9.DOI:10.16333/j.1001-6880.2017.4.024
Plackett-Burman和Box-Behnken试验设计优化超声波-酶法提取青钱柳多糖工艺及结构初探
Optimization of Ultrasonic and Enzyme-assisted Extraction of Polysaccharides from Cyclocarya paliurus by Plackett-Burman and Box-Behnken Experiment and Analysis of Its Structure
摘要
Abstract
To develop the resource of Cyclocarya paliurus polysaccharides (CPP),ultrasonic and enzyme-assisted extraction of CPP was optimized and its structure was preliminarily studied.Firstly,the optimum range of each single factor was identified by single factor experiment.Then,the key factors affecting the yield of CPP were screened with Plackett-Burman design,and the extraction of CPP was optimized with Box-Behnken experiment.Finally,UV,FTIR spectra and gas chromatograms were employed to preliminarily identify the structure of CPP.Results indicated that the key factors were pH,amount of mixed enzyme,enzymatic time and ultrasonic power.In addition,the optimal extraction conditions were pH of 6.11,amount of mixed enzyme of 4.81％,enzymatic time of 1.685 h,ultrasonic power of 96.9 W,solid to liquid ratio of 1∶20,and ultrasonic time of 30 min.Under these conditions,the extraction yield of CPP was 7.18％.CPP was a typical polysaccharide and the monosaccharide composition was consisted of rhamnose,arabinose,xylose,mannose,glucose,galactose,with a molar ratio of 1.00∶ 7.03∶ 2.06∶2.11 ∶ 5.25∶ 12.06.关键词
青钱柳多糖/超声波/复合酶/Plackett-Burman试验设计/Box-Behnken试验设计/单糖组成Key words
Cyclocarya paliuruspolysaccharides/ultrasonic/mixed enzyme/Plackett-Burman experiment/Box-Behnken experiment/monosaccharide composition分类
轻工纺织引用本文复制引用
胡文兵,杨占威,陈慧,熊磊,彭俊,王文君..Plackett-Burman和Box-Behnken试验设计优化超声波-酶法提取青钱柳多糖工艺及结构初探[J].天然产物研究与开发,2017,29(4):671-679,9.基金项目
国家自然科学基金(31560459,31160319) （31560459,31160319）
江西省自然科学基金(20151BAB204037) （20151BAB204037）
江西省高等学校科技落地计划项目(KJLD13027) （KJLD13027）
江西省教育厅科技计划项目(GJJ13281) （GJJ13281）
