西北林学院学报2017,Vol.32Issue(3):116-121,6.DOI:10.3969/j.issn.1001-7461.2017.03.22
不同生长调节剂对蒙古栎嫩枝扦插生根的影响
Cuttage Propagation Technique and Rooting Mechanism of Quercus mongolica with Softwood Cutting
摘要
Abstract
In order to establish an efficient cutting propagation system,the rooting mechanism of softwood cuttings of Quercus mongolica was explored.The current year shoots in the middle and upper branches of Q.mongolica were used as cuttings,which were treated by different growth regulators of ABT No.1,IBA,NAA,and mixed IBA+NAA with gradient concentrations of 200,300,500 mg · L-1,and 1 000 mg · L-1for different time durations,and water was used as the control.Four endogenous hormones,i.e.,indoleacetic acid (IAA),abscisic acid (ABA),zeatin (ZT),and gibberellin (GA3) during rooting were determined by high performance liquid chromatography (HPLC).The results showed that the treatment with IBA 1 000 mg · L-1 for 10 s and IBA+NAA 1 000 mg · L-1 for 10 s could promote the callus development,but the rooting rate was only 3.3%.In the process of rooting,IAA and ZT could promote the development of callus and promote the rooting.ABA inhibited the rooting,while low level of GA3 was beneficial to adventitious root formation.There was a positive correlation between IAA/ABA ratio and ZT/ABA ratio in callus growth and rooting ability.关键词
蒙古栎/扦插/繁殖/生长调节剂Key words
Quercus mongolica/cuttage propagation/growth regulator分类
农业科技引用本文复制引用
闫文涛,佟兆庆,魏俊,康德星,陆秀君..不同生长调节剂对蒙古栎嫩枝扦插生根的影响[J].西北林学院学报,2017,32(3):116-121,6.基金项目
辽宁省农业科技攻关项目(2014207005/2015207005). (2014207005/2015207005)