新疆农业科学2017，Vol.54Issue(4)：589-596,8.DOI:10.6048/j.issn.1001-4330.2017.04.001
不同含油率核桃品种种仁主要营养物质含量和糖代谢相关酶活性时节动态
The Seasonal Dynamics of Main Nutrients' Content and Sugar Metabolizing Enzyme Activities in Kernel of Walnut Varieties with Different Oil Contents
摘要
Abstract
[Objective] Through the analysis of seasonal dynamics of crude fat content, crude protein content and soluble sugar content and the activities of enzymes related with sugar metabolism in the kernel of walnut varieties with different oil contents, this study aimed at understanding the relationship between oil content and nutrition metabolism and having a deep insight into the metabolism of oil synthesis in walnut kernel.[Method]Walnut varieties with high oil content (Juglans.regia'Xinfeng'and J.regia'Wen185') and walnut varieties with low oil content (J.regia'Xinzaofeng', J.regia'Zha 343'and J.regia'Xinxin2') were used as the experimental materials.In the developmental process of walnut fruit, the walnut kernels with different oil contents were sampled to measure the content of crude fat, crude protein and soluble sugar, and the activities of enzymes related with sugar metabolism.[Result]120-150 days after flowering (DAF), there was a significant difference in crude fat content in kernel among different walnut varieties (P<0.05).60 DAF, the soluble sugar content in walnut varieties with high oil content was significantly higher than that in walnut varieties with low oil content (P<0.05).120-150 DAF, the soluble sugar content in walnut varieties with high oil content was significantly lower than that in walnut varieties with low oil content (P<0.05).There was no regular difference in crude protein content in kernel between walnut varieties with different oil content.30-60 DAF, the activities of AI and NI in the walnut varieties with high oil content were significantly lower than those in the walnut varieties with lower oil content (P<0.05).120-150 DAF, the activity of SPS in walnut varieties with high oil content was significantly higher than that in low oil content varieties (P<0.05).But there was no regular difference in the activity of SS in kernel between walnut varieties with different oil contents.[Conclusion]The oil synthesis in the walnut kernel is neither dependent on the early accumulation of soluble sugar in the kernel nor dependent on protein metabolism, but the low activities of NI and AI in kernel during the early stage of walnut fruit development and the high activities of SPS during later stage are conducive to the oil synthesis.关键词
核桃/种仁/含油率/营养物质/糖代谢相关酶Key words
walnut/kernel/oil content/nutrients/sugar metabolizing enzyme分类
农业科技引用本文复制引用
张翠芳,潘存德,陈虹,史彦江..不同含油率核桃品种种仁主要营养物质含量和糖代谢相关酶活性时节动态[J].新疆农业科学,2017,54(4):589-596,8.基金项目
国家自然科学基金项目"新疆早实核桃主栽品种坚果种仁油脂亏缺成因及其研究"(31460210) （31460210）
新疆农业大学产学研联合培养研究生示范基地项目"不同含油量核桃种品质差异的研究"(XJAUCXY-YJS-20152013)Supported by National Natural Science Foundation of China "The cause and mechanism of Xiniang precocious walnut cultivar kernel oiliness" (31460210) and Demonstration Base Project of Graduates Cultivation of Enterprise-University-Research Institute Cooperation of Xinjiang Agricultural University "Research on quality differences in kernel of walnut varieties with different oil content" (XJAUCXY-YJS-20152013) （XJAUCXY-YJS-20152013）
