新疆农业科学2017,Vol.54Issue(4):605-617,13.DOI:10.6048/j.issn.1001-4330.2017.04.003
枸杞幼苗对NaCl和Na2CO3胁迫的生理响应
Physiological Response of Lycium bararum L.Seedling to NaCl and Na2CO3 Stres
摘要
Abstract
[Objective] The present study aims to understand the salt tolerance of two kinds of Chinese wolfberry seedling and provide the theoretical basis for the large-scale popularization and the utilization of saline-alkali land and sandy wasteland of Chinese wolfberry.[Method]Using the seedlings of Lycium ruthenicum and Lycium barbarum as test materials, watered with NaCl and Na2CO3 solution to study the effects of salt stress on seedlings physiological characteristics of Lycium bararum L.[Result]The results showed that with the increase of NaCl and Na2CO3 concentration, the cell membrane permeability, malondialdehyde, proline and soluble sugar content increased significantly in different time points, Soluble protein content and SOD, POD, CAT enzyme activity showed an upward trend in the early stage of treatment (10 d-30 d), the later treatment showed a trend of increasing and then decreasing t (40 d-50 d).With the increase of NaCl and Na2CO3 processing days, under the same salt concentration, the cell membrane permeability, malondialdehyde, proline and soluble sugar content increased significantly, the soluble protein content and SOD, POD enzyme activity showed a trend of increasing first and then decreasing, and the activity of CAT enzyme showed a downward trend.The effects of Na2CO3 stress on seedling were more significant than that of NaCl stress, the change trend of Lycium chincnse Ningqi 7 was higher than Lycium chincnse Heiqi 1, and with the increase of treatment concentration and treatment days, the degree of injury was increased significantly.[Conclusion]Under the stresses of NaCl and Na2CO3 in the two varieties Chinese wolfberry, malondialdehyde, proline, soluble sugar and the activity of antioxidant enzyme SOD are more sensitive and could be used as the main index to evaluate the salt tolerance of Lycium barbarum.In addition, the seedlings of Lycium chincnse Heiqi 1 and Lycium chincnse Ningqi 7 reached the threshold value at 1.2 mol/L and 1.0 mol/L and the limit value of salt tolerance at 1.5 mol/L under the stress of NaCl, while reached the threshold value at 0.2 mol/L and 0.1 mol/L and the limit value of salt tolerance at 0.5 and 0.4 mol/L under the stress of Na2CO3, which implicated that Lycium chincnse Heiqi 1 had the stronger salt tolerance than Lycium chincnse Ningqi 7, and the effect of Na2CO3 on the seedlings of Chinese wolfberry was significantly greater than that of NaCl.关键词
枸杞/盐胁迫/叶片/生理特性Key words
Lycium bararum L./Salt stress/Leaves/Physiological characteristics分类
农业科技引用本文复制引用
齐延巧,孙静芳,赵世荣,李勇,王建友,王琴,廖康..枸杞幼苗对NaCl和Na2CO3胁迫的生理响应[J].新疆农业科学,2017,54(4):605-617,13.基金项目
中央财政林业科技推广项目-新疆枸杞高效栽培技术示范与推广 ()
新疆维吾尔自治区园艺学重点学科建设基金 Forestry science and technology promotion projects of the central government finance of China "Demonstration and popularization of high quality and efficient cultivation technique of Chinese wolfberry in Xinjiang" and Key discipline (horticulture) fund of Xinjiang Uyghur Autonomous Region (horticulture)