西南林业大学学报2017，Vol.37Issue(2)：60-68,9.DOI:10.11929/j.issn.2095-1914.2017.02.010
菊花黄化突变体初步研究
The Preliminary Study of Chlorina Mutant in Dendranthema morifolium
摘要
Abstract
The photosynthetic pigment content and de novo RNA-Seq were investigated in leaf-color mutant and normal plant of Dendranthema morifolium'Xiaguang Feiyue'.The results showed that the photosynthetic pigment content had decreased greatly of mutant plants,only about 18 percent of normal plants,but the Chl a/b observably increased in mutant plant.We generated 4.75 Gb and 4.96 Gb clean datum from the mutant and normal plant leaves using transcriptome sequencing,respectively.De novo assembly yielded 105 456 unigenes with 15 493 of length greater than 1 kb,1 556 bp of N50.For unigenes expression analysis,we got 3 762 differentially expressed genes (DEGs),and obtained 2 741 annotations by functional annotation of the DEGs.Using GO and KEGG enrichment analyses,and qPCR verification,results indicated that forming reason of the chlorina mutant of D.morifolium 'Xiaguang Feiyue'may be expressed at low level of dmGLK2 and dmGLK1 not expressed cause chloroplast development and division blocked,chloroplast number is greatly reduced in leaf cells,thus the content of chlorophyll is overall declined.And the study will supply meaning information to the future studies on leaf-color mutants and directional breeding of D.morifolium.关键词
菊花/突变体/无参转录组测序/差异基因Key words
Dendranthema morifolium/mutant/de novo RNA-Seq/DEGs分类
农业科技引用本文复制引用
卢珍红,莫锡君,蒋亚莲,余蓉培,周旭红,田敏,杨晓,桂敏..菊花黄化突变体初步研究[J].西南林业大学学报,2017,37(2):60-68,9.基金项目
云南省重大科技专项课题二(2016ZA006)资助. （2016ZA006）
