西南林业大学学报2017,Vol.37Issue(2):74-79,6.DOI:10.11929/j.issn.2095-1914.2017.02.012
大量元素对多花黄精不定芽生长和次生代谢物含量的影响
Effects of Macroelement Concentration on Growth and Secondary Metabolites Contents of Polygonatum cyrtonema's Adventitious Bud
摘要
Abstract
Taken germinative rootstock of Polygonatum cyrtonema as the material.And the experiment was based on MS + NAA 1.0 mg/L+ KT 1.5 mg/L medium.It was investigated the complex effect that made by the concentration of the 5 kinds of macroelements in MS medium on the growth of germinative rootstock of P.cyrtonema and the 2 kinds of secondary metabolites include amylose and total saponins.The results indicated that in the most suitable medium for the growth of P.cyrtonema germinative rootstock,the concentration of NH4NO3 and KNO3 was 1.5 times as strong as the standard concentration of MS medium.The concentration of K-H2PO4,MgSO4 and CaC12 was 2 times as strong as the standard concentration of MS medium.In the most suitable medium that synthesized by total anylose,the concentration of KH2PO4 was 2 times as strong as the standard concentration of MS medium.In addition,the other elements were all as strong as the standard concentration of MS medium.In the most suitable medium that synthesized by total saponins,the concentration of 5 kinds of macroelements were MS medium's standard concentration.关键词
多花黄精/组织培养/大量元素/次生代谢物/根茎芽Key words
Polygonatum cyrtonema/tissue culture/macroelement/secondary metabolites/adventitious bud分类
农业科技引用本文复制引用
周新华,肖智勇,黄拯,曾满生,李峰卿,武晓玉..大量元素对多花黄精不定芽生长和次生代谢物含量的影响[J].西南林业大学学报,2017,37(2):74-79,6.基金项目
中央级公益性科研院所基本科研业务费专项资金项目(CAFYBB2017MB024)资助. (CAFYBB2017MB024)