中风与神经疾病杂志2017，Vol.34Issue(5)：398-405,8.
瞬时感受电位香草酸亚家族蛋白1受体在慢性偏头痛大鼠模型中的作用研究
The role of Transient Receptor Potential Vanilloid subfamily member-1 in chronic migraine rat model
摘要
Abstract
Objective In this study,the model rats with chronic inflammation(chronic migraine,CM)was established by repeated stimulation of the superior sagittal sinus of SD rats with inflammatory soup(IS).In order to study the pathogenesis of chronic migraine(CM)and provide theoretical basis for the treatment of drugs,to explore the role of Transient Receptor Potential Vanilloid subfamily member-1(TRPV1)receptor in the pathogenesis of CM.Methods Seventy-two Rats were randomly divided into four groups:blank control group(A group),sham operation group(B group),CM group(C group),Capsazepine group(D group).The dura mater,trigeminal ganglion(TG)and spinal trigeminal nucleus caudalis(TNC)were dissected to detect the expression of TRPV1 receptor and Calcition Gene-related Peptide(CGRP)by immunohistochemistry,Western blot and real-time PCR.Results The expression levels of TRPV1 receptor and CGRP in dura mater,TG and TNC were increased in CM rats(P<0.05),and the behavioral of rats was significantly reduced in rats after intracerebroventricular injection of Capsazepine,and the expression of TRPV1 receptor and CGRP were decreased(P<0.05).Conclusion TRPV1 receptor is involved in CM neurogenic inflammatory response and pain transmission by affecting CGRP release,and the results suggest that TRPV1 maybe involved in the pathophysiology of CM via TRPV1-CGRP signaling pathway.关键词
慢性偏头痛/瞬时感受电位香草酸亚家族蛋白1受体/降钙素基因相关肽/三叉神经血管系统/机械刺激缩足反应阈值/拮抗剂Key words
Chronic migraine/Transient Receptor Potential Vanilloid subfamily member-1/Calcition gene-related peptide/Trigenmi-novascular system/Paw withdrawal mechanical threshold/Antagonist分类
临床医学引用本文复制引用
宋晓文,陈金波,吴欣彤,李斌,徐文香,鲁文先,董晓梦,苏毅鹏..瞬时感受电位香草酸亚家族蛋白1受体在慢性偏头痛大鼠模型中的作用研究[J].中风与神经疾病杂志,2017,34(5):398-405,8.基金项目
山东省高等学校科技计划项目(No.J12LL63) （No.J12LL63）
