中国动脉硬化杂志2017，Vol.25Issue(5)：475-479,5.
miR-142-5p靶向调控TGF-β2促进人巨噬细胞凋亡
MiR-142-5p target regulating TGF-β2 promotes human macrophage apoptosis
李玉东 1杨清泉 1张明磊 1王星 1刘慧 1李聪聪1
作者信息
- 1. 郑州大学附属南阳中心医院特需病房一病区,河南省南阳市473000
- 折叠
摘要
Abstract
Aim To study the expression of miR-142-5p in atherosclerosis tissues and its effect on human macrophage apoptosis.Methods Rat atherosclerosis model was constructed,qRT-PCR was used to detect the expression of miR-142-5p in atherosclerotic tissues.50 μg/L ox-LDL stimulated human macrophage,vascular smooth muscle cell,endothelial cell for 24 h,qRT-PCR was used to detect the expression of miR-142-5p in the cells.Target gene prediction software was used to predict the target gene of miR-142-5p,dual luciferase reporter gene was used to identify the target gene.Cells were transfected with miR-142-5p mimic,mimic control,miR-142-5p inhibitor,inhibitor control into macrophage,Western blot and qRT-PCR were used to to detect the expression of miR-142-5p on target gene,the effect of miR142-5p and target gene on the apoptosis of macrophages was detected by flow cytometry.Results MiR-142-5p expression was up-regulated in atherosclerotic tissues compared with normal tissue,and the difference was significant (P＜0.01).Mter stimulation with ox-LDL,the levels of miR-142-5p in vascular smooth muscle cells and endothelial cells did not change significantly compared with before stimulation,but the level of miR-142-5p in the macrophages was significantly higher than that before stimulation (P＜0.01).Predicting the target gene of miR-142-5p was transforming growth factor-β2 (TGF-β2).The luciferase activity was the lowest after transfection with miR-142-5p mimic and TGF-β2.The expression of TGF-β2 protein and mRNA in miR-142-5p mimic group was significantly decreased compared with mimic control group (P＜0.01),the expression of TGF-β2 protein and mRNA in miR-142-5p inhibitor group was significantly higher than that in inhibitor control group (P＜0.01),the apoptosis rate of miR-142-5p mimic group was significantly higher than that of mimic control group (P＜0.01),the apoptosis rate of TGF-β2 siRNA group was significantly higher than that of siRNA control group (P＜0.01).Conclusion MiR-142-5p was over expressed in atherosclerosis tissues,and miR-142-5p could promote the apoptosis of human macrophages by regulating the target gene TGF-β2.关键词
动脉粥样硬化/靶基因/miR-142-5p/细胞凋亡Key words
Atherosclerosis/Target gene/MiR-142-5p/Apoptosis分类
医药卫生引用本文复制引用
李玉东,杨清泉,张明磊,王星,刘慧,李聪聪..miR-142-5p靶向调控TGF-β2促进人巨噬细胞凋亡[J].中国动脉硬化杂志,2017,25(5):475-479,5.
