中国药房2017，Vol.28Issue(16)：2198-2201,4.DOI:10.6039/j.issn.1001-0408.2017.16.10
清脑片对大鼠脑缺血再灌注损伤的预防作用
Preventive Effect of Qingnao Tablet on Cerebral Ischemia-reperfusion Injury in Rats
摘要
Abstract
OBJECTIVE:To study the preventive effect of Qingnao tablet on cerebral ischemia-reperfusion injury in rats. METHODS:Rats were randomly divided into sham operation group,model group,Naoluotong capsule group (positive control, 0.05 g/kg),Qingnao tablet high-dose,medium-dose,low-dose groups(1.52,0.76,0.38 g/kg),10 in each group. Rats in all ad-ministration groups were intragastrically given relevant medicines,rats in sham operation group and model group were intragastrical-ly given equal volume of sodium carboxymethylcellulose solution,once a day,for 5 d. After 1 h of last administration,all rats were induced for cerebral ischemia-reperfusion injury model by suture-occluded method except for sham operation group. After 22 h of ischemia-reperfusion,neurological function deficit scoring was conducted;the pathological changes of the hippocampus were ob-served;superoxide dismutase (SOD),adenosine triphosphate (ATP),lactate dehydrogenase (LDH),tumor necrosis factor α(TNF-α)levels in brain tissue were measured. RESULTS:Compared with sham operation group,rats in model group appeared dif-ferent degrees of neurological deficits(score declined),sparse neurons,irregularly arranged in hippocampus as well as other patho-logical changes;ATP,SOD levels in brain tissue were decreased(P<0.01),LDH,TNF-α levels were increased(P<0.01). Com-pared with model group,neurological function deficit scores in Qingnao tablet doses groups were increased(P<0.05),neurologi-cal deficits were improved. Except for sham operation group,brain tissue indexes in other administration groups were significantly improved(P<0.05 or P<0.01). CONCLUSIONS:Qingnao tablet can increase ATP and SOD levels in brain tissue homogenate of model rats with cerebral ischemia-reperfusion,decrease LDH and TNF-α levels,and obviously improve rats'cerebral ischemia-re-perfusion injury.关键词
清脑片/脑缺血再灌注损伤/大鼠/预防作用Key words
Qingnao tablet/Cerebral ischemia-reperfusion injury/Rat/Preventive effect分类
医药卫生引用本文复制引用
黎丹东,谢国旗,高延玲,苏峰,王希东,李艳,张正臣..清脑片对大鼠脑缺血再灌注损伤的预防作用[J].中国药房,2017,28(16):2198-2201,4.基金项目
河南新乡重点科技攻关项目(No.CXGG16054) （No.CXGG16054）
