中国药房2017，Vol.28Issue(16)：2220-2223,4.DOI:10.6039/j.issn.1001-0408.2017.16.16
黎药益智仁不同极性部位提取物对实验性肠炎模型小鼠的影响
Effects of Extracts from Different Polar Parts of Li Medicine Alpinia oxyphylla Fruit on Model Mice with Experimental Colitis
摘要
Abstract
OBJECTIVE:To investigate the effects of 4 polar parts extracts from Li medicine Alpinia oxyphylla fruit on model mice with experimental colitis,and screen the effective parts of A. oxyphylla fruit in the treatment of colitis. METHODS:After 70% ethanol extract dispersed by water,petroleum ether,trichloromethane,ethyl acetate and n-butanol were used in turn to obtain extractions in related parts. 42 mice were randomly divided into modeling group(36 mice)and blank group(6 mice). Mice in mod-eling group were taken 2,4,6-nitrobenzne sulfonic acid method to replicate the experiment colitis. After modeling,model mice were randomly divided into model group,positive group(Sulfasalazine enteric coated tablets,52 g/kg),petroleum ether,trichloro-methane,ethyl acetate,n-butanol extract from A. oxyphylla fruit groups(10 g/kg,calculated by crude drug),6 in each group,in-tragastrically administrated once a day,for 9 d,0.2 mL/10 g;mice in blank group and model group were intragastrically given nor-mal saline. After administration,body mass of mice was determined,disease activity index(DAI)was scored and colonic myelo-peroxidase(MPO),superoxide dismutase(SOD),malondialdehyde(MDA)activities in colon tissue were detected,and the colon pathological changes were observed and scored. RESULTS:Compared with blank group,decrease percentage of body mass,DAI score,MPO and MDA activities in colon tissue in model group were increased(P<0.05 or P<0.01);colon tissue was pathologi-cally changed. Compared with model group,body mass was increased,DAI score,MPO activity and pathological score in each ad-ministration groups were decreased;SOD activities in positive group,n-butanol extract from A. oxyphylla fruit group and ethyl ace-tate extract from A. oxyphylla fruit group were significantly increased,MDA activities in positive group and ethyl acetate extract from A. oxyphylla fruit group were significantly decreased,with statistical significances (P<0.05 or P<0.01). CONCLUSIONS:The 4 polar parts extracs from A. oxyphylla fruit have certain improvement effects on model mice with experimental colitis, in which ethyl acetate and n-butanol parts are preferred.关键词
益智仁/提取部位/实验性肠炎/小鼠Key words
Alpinia oxyphylla fruit/Extraction parts/Ex-perimental colitis/Mice分类
医药卫生引用本文复制引用
房磊臣,刘嫱,高新征,韦祎,刘一鸣,黄凌..黎药益智仁不同极性部位提取物对实验性肠炎模型小鼠的影响[J].中国药房,2017,28(16):2220-2223,4.基金项目
国家自然科学基金资助项目(No.81360586, 81473618) （No.81360586, 81473618）
海南省自然科学基金资助项目(No.812186,814290) （No.812186,814290）
海南省中药现代化专项项目(No.ZY201332) （No.ZY201332）
海口市重点科技计划项目(No.2013-50) （No.2013-50）
