中国医科大学学报2017，Vol.46Issue(5)：392-396,400,6.DOI:10.12007/j.issn.0258⁃4646.2017.05.003
白细胞介素17A在急性百草枯中毒小鼠肾损伤中的作用
Effects of Interleukin?17A on Acute Paraquat?intoxication?induced Kidney Injury in Mice
摘要
Abstract
Objective To investigate the effects of interleukin?17A on kidney injury induced by paraquat(PQ). Methods Seventy?two ICR mice were randomly divided into 3 groups:NS,PQ,and PQ+Ab (n=24 for each). The PQ?poisoning model was established by administering a gavage of PQ solution;mice in the PQ+Ab group were then administereda dose of anti?IL?17A antibody 2 hours later by i.p. injection,whereas the NS group were administered a corresponding volume of normal saline instead.The mice were killed at 8,24,48,or 72 h to obtain renal tissues and serum. An enzyme?linked immunosorbent assay(ELISA)was used to determine serum IL?17A,serum creatinine(SCr),and blood urea nitrogen (BUN)levels.Chemical colorimetry was used to detect the viability of myeloperoxidase(MPO )in renal tissue,and hematoxylin?eosin(HE)stain?ing was used to observe the renal pathologic changes. Immunohistochemistry(IHC)and PCR were used to examine IL?17A expression in renal tis?sues. Results Serum IL?17A,renal tissue MPO viabilities,BUN,and SCr were increased in the PQ and PQ+Ab groups,compared to those in the NS group(P<0.01). However,the above?mentioned parameters were lower in the PQ+Ab group than in the PQ group(P<0.01). Conclusion IL?17A promotes mouse kidney injury induced by acute PQ?intoxication through activating and/or recruiting neutrophils;therefore,blockade IL?17A,with antibody can attenuate the injury.关键词
白细胞介素17A/百草枯/中毒/肾损伤/小鼠Key words
interleukin⁃17A/paraquat/intoxication/kidney injury/mice分类
医药卫生引用本文复制引用
宋宏伟,杨琛,刘伟,刘志..白细胞介素17A在急性百草枯中毒小鼠肾损伤中的作用[J].中国医科大学学报,2017,46(5):392-396,400,6.基金项目
国家自然科学基金(81571882) （81571882）
辽宁省科学技术计划(2013225303) （2013225303）
