中国医科大学学报2017，Vol.46Issue(6)：481-484,4.DOI:10.12007/j.issn.0258⁃4646.2017.06.001
Akt和SRC在Exosomes促进同源肺癌细胞增殖中的作用
Role of Akt and SRC Pathways in Exosome?mediated Proliferation of Homologous Lung Adenocarninoma Cells
摘要
Abstract
Objective To explore the effect of Exosomes isolated from the A549 lung cancer cells on the proliferation of these cells and their ho?mologous tumor cells,HCC827,and the role of the PI3K/Akt and SRC signaling pathways in this process. Methods Exosomes were isolated from the supernatant after density gradient centrifugation of A549 cells. The Exosomes morphology was observed by transmission electron microscopy. The expression of the Exosome?specific proteins was analyzed using Western blotting. Cell proliferation was investigated using the MTT assay. Re?sults The A549?derived Exosomes were 30?100 nm in diameter and had a bilayer membrane.Western blotting showed that CD9 was detected in these Exosomes. The isolated Exosomes promoted the proliferation of the A549 and the HCC827 cells in a dose?and time?dependent manner,ac?companied by the activation of Akt and SRC. Conclusion Exosomes isolated from A549 cells promote the proliferation of the secreting cells and the homologous tumor cells in a dose?and time?dependent manner. The mechanism may be related to the activation of Akt and SRC.关键词
Exosomes/增殖/肺癌/Akt/SRCKey words
Exosomes/lung cancer/proliferation/Akt/SRC分类
医药卫生引用本文复制引用
解世林,胡雪君,曲晶磊,范一博,车晓芳,侯科佐,曲秀娟,刘云鹏,王晓楠,康健..Akt和SRC在Exosomes促进同源肺癌细胞增殖中的作用[J].中国医科大学学报,2017,46(6):481-484,4.基金项目
国家自然科学基金(81372546,81472193) （81372546,81472193）
辽宁省科学技术计划(2014226033,2014225013,L2014296) （2014226033,2014225013,L2014296）
辽宁省教育厅重点实验室基础研究项目(LZ2014037) （LZ2014037）
