中国医科大学学报2017，Vol.46Issue(6)：495-500,6.DOI:10.12007/j.issn.0258⁃4646.2017.06.004
Pin1与CyclinD1在胃肠间质瘤中的表达及临床意义
Expression and Clinical Significance of Pin1 and CyclinD1 in Gastrointestinal Stromal Tumor
摘要
Abstract
Objective To investigate the relationship between Pin1 and CyclinD1 expression and the development of gastrointestinal stromal tu?mor(GIST). Methods The protein and mRNA expression of Pin1 and CyclinD1 in 85 samples of GIST and adjacent non?cancerous tissues were detected by immunohistochemistry and real?time quantitative polymerase chain reaction. Results The expression rate of Pin1 protein in GIST tis?sues(64.7%;55/85)was higher than that in adjacent non?cancerous tissues(26.7%;4/15). Similarly,the expression rate of CyclinD1 protein in GIST tissues(42.3%;36/85)was higher than that in adjacent non?cancerous tissues(6.7%;1/15). The expression of Pin1 and CyclinD1 mRNA in GIST tissues was 7.03 and 5.53 times that in adjacent non?cancerous tissues ,respectively. There was no obvious correlation between the expres?sion of Pin1 and clinicopathological parameters. The expression of CyclinD1 was positively correlated with the grade of NIH and tumor diameter (P<0.05). There was a significant correlation between the expression of Pin1 and CyclinD1 in GIST tissues. Conclusion The expression of both Pin1 and CyclinD1 was up?regulated in GIST tissues. The significant correlation between the expression of Pin1 and CyclinD1 in GIST tissues sug?gests that their synergistic effect promotes carcinogenesis and the development of GIST.关键词
胃肠间质瘤/Pin1/CyclinD1/免疫组化/实时荧光定量PCRKey words
gastrointestinal stromal tumor/Pin1/CyclinD1/immunohistochemistry/real⁃time quantitative polymerase chain reaction分类
医药卫生引用本文复制引用
黄子成,陈清,翁晓源,杨振荣,陈惠忠,周永建..Pin1与CyclinD1在胃肠间质瘤中的表达及临床意义[J].中国医科大学学报,2017,46(6):495-500,6.基金项目
国家自然科学基金(81172380) （81172380）
福建省卫生系统学术技术带头人后备人基金(010114201) （010114201）
国家临床重点专科建设基金[卫办医政函(2012)649号] （2012）
