中国医科大学学报2017，Vol.46Issue(6)：510-514,518,6.DOI:10.12007/j.issn.0258⁃4646.2017.06.007
应用iTRAQ技术筛选先天性心脏畸形胎儿母亲血清差异表达蛋白
The Identification of Differentially Expressed Proteins in Maternal Serum of Fetuses with Congenital Heart Defects by Using the iTRAQ Technology
摘要
Abstract
Objective To screen for serum protein differentially expressed between women whose fetuses had congenital heart defects(CHD) and women who had normal fetuses. Methods Serum samples were collected from pregnant women whose fetuses had CHD and those whose fe?tuses had no CHD,including a CHD group of 40 women and a control group of 10 women. The CHD group included 4 subgroups as follows:tetralo?gy of Fallot,ventricular septal defects,persistent truncus arteriosus,and a mixture of relatively rare types of CHD(n=10 each). Samples in the same group were pooled to obtain equal amounts of proteins ,and the iTRAQ proteomic approach was used to identify and quantify the proteins dif?ferentially expressed among these groups. Results We successfully identified 606 proteins,among which 47 showed at least a 1.5?fold difference between the CHD and control groups. Among the 47 proteins,23 and 24 were upregulated and downregulated,respectively. Conclusion Several proteins associated with CHD could be identified by using the iTRAQ proteomic approach ,and various proteins were involved in the pathogenesis of CHD in this study.关键词
同位素标记相对和绝对定量技术/先天性心脏畸形/蛋白质组学/血清标志物/产前诊断Key words
isobaric tag for relative and absolute quantitation/congenital heart defect/proteomics/serum biomarker/prenatal diagno⁃sis分类
医药卫生引用本文复制引用
陈骊珠,马巍,张墨,袁正伟..应用iTRAQ技术筛选先天性心脏畸形胎儿母亲血清差异表达蛋白[J].中国医科大学学报,2017,46(6):510-514,518,6.基金项目
国家自然科学基金青年基金项目(81600258) （81600258）
国家重点研发计划(2016YFC1000505) （2016YFC1000505）
国家自然科学基金(81671469) （81671469）
