中国医科大学学报2017，Vol.46Issue(6)：515-518,4.DOI:10.12007/j.issn.0258⁃4646.2017.06.008
益气健脾方对脾气虚证模型大鼠心肌细胞线粒体呼吸链酶复合物活性的影响
Effect of a Decoction to Nourish Qi and Invigorate the Spleen on the Activity of Mitochondrial Respiratory Chain Enzyme Complexes in Cardiomyocytes of Rats with Spleen Qi Deficiency Syndrome
摘要
Abstract
Objective To investigate the effect of a decoction to nourish qi and invigorate the spleen on mitochondrial respiratory chain enzyme complex activity in cardiomyocytes of rats with spleen qi deficiency syndrome. Methods Rats were randomly divided into normal,model,and treatment groups. The model and treatment groups were treated by diet intervention combined with the limit swim method. The general condition and spleen qi deficiency syndrome were assessed on day 15. After the success of the model,the normal and model groups were treated with a con?ventional feeding method combined with normal saline ,and the treatment group was treated by diet intervention combined with a decoction to nour?ish qi and invigorate the spleen for 9 weeks. The activity of two mitochondrial respiratory chain enzyme complexes was observed. Results The ac?tivity of mitochondrial respiratory chain enzyme complexⅡand complexⅣin the model group was significantly lower than the activity in the nor?mal and treatment groups(P<0.05). The activity levels of complexⅡand complexⅣwere significantly different between the model group and the treatment group(P<0.05). Conclusion Spleen qi deficiency can cause decreased activity of mitochondrial respiratory chain enzyme com?plexes in myocardial cells. The decoction to nourish qi and invigorate the spleen can modulate the activity of myocardial mitochondrial respiratory chain enzyme complexesⅡandⅣ.关键词
益气健脾/脾气虚/线粒体/呼吸链酶复合物Key words
nourishing qi to invigorate spleen/spleen qi deficiency/mitochondria/respiratory chain enzyme complex分类
医药卫生引用本文复制引用
李思琦,张哲,杨关林,宋囡,闵冬雨,王凤荣..益气健脾方对脾气虚证模型大鼠心肌细胞线粒体呼吸链酶复合物活性的影响[J].中国医科大学学报,2017,46(6):515-518,4.基金项目
辽宁省教育厅科学技术研究一般项目(L2014365) （L2014365）
