中国医科大学学报2017，Vol.46Issue(6)：552-556,5.DOI:10.12007/j.issn.0258⁃4646.2017.06.017
姜黄素联合顺铂对人舌鳞癌Tca⁃8113细胞的影响
Effect of Curcumin Combined with Cisplatin on Human Tongue Squamous Cell Carcinoma Tca?8113 Cells
佟周 1刘长富 1谭婧玉 1王稚英1
作者信息
- 1. 锦州医科大学附属第二医院口腔颌面外科,辽宁 锦州 121001
- 折叠
摘要
Abstract
Objective To observe the effect of curcumin combined with cisplatin on the proliferation of human tongue squamous cell carcinoma Tca?8113 cells,and explore the possible mechanism of their anti?tumor effect in vitro. Methods In this study,the samples were divided into four groups:curcumin,cisplatin,curcumin combined with cisplatin,and control. Detection of rate of inhibition of Tca?8113 cell proliferation was carried out by MTT assay. This was followed by observation of the morphological changes of the nuclei by Hoechst 33258 fluorescence staining. Subse?quently,cellular apoptosis was assayed by flow cytometry,and expression of Notch1 and epidermal growth factor receptor(EGFR)was examined by Western blotting. Results Results of MTT assay showed that curcumin combined with cisplatin inhibited the proliferation of tongue squamous cell carcinoma to a greater extent than either curcumin or cisplatin alone(P<0.05). Flow cytometry analysis indicated that,unlike curcumin or cisplatin alone,curcumin combined with cisplatin noticeably induced apoptosis(P<0.05). Results of Western blotting revealed that the expres?sion of Notch1 was upregulated,whereas the expression of EGFR was downregulated to a greater extent in the control group than in cells treated with curcumin or cisplatin alone. Unlike curcumin and cisplatin groups,the combined group revealed statistically significant expressions of Notch1 and EGFR(P<0.05). Conclusion Curcumin and cisplatin have a combined effect on inhibition of proliferation of human tongue squamous cell carcinoma Tca?8113 cells. The mechanism may be related to upregulation of the Notch1 signaling pathway and downregulation of the EGFR signal?ing pathway.关键词
姜黄素/顺铂/人舌鳞癌Tca⁃8113细胞/细胞凋亡/信号通路Key words
curcumin/cisplatin/human tongue squamous cell carcinoma Tca⁃8113 cells/cell apoptosis/signal pathway分类
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佟周,刘长富,谭婧玉,王稚英..姜黄素联合顺铂对人舌鳞癌Tca⁃8113细胞的影响[J].中国医科大学学报,2017,46(6):552-556,5.
