中国医科大学学报2017，Vol.46Issue(6)：561-565,5.DOI:10.12007/j.issn.0258⁃4646.2017.06.019
RNF180启动子区甲基化在前列腺癌中的作用机制
RNF180 Promoter Methylation in Prostate Cancer
王海光 1姜华茂 1左知润 1龙湟哲 1袁观远 1贾凡振1
作者信息
- 1. 锦州医科大学附属第一医院泌尿外科,辽宁 锦州 121001
- 折叠
摘要
Abstract
Objective To clarify the significance of RNF180 expression in carcinogenesis and progression of prostate cancer by detection of RNF180 promoter methylation. Methods RNF180 expression was detected in human prostate cancer cell lines(PC3,LNCap,and DU145)and normal prostate cells(RWPE?1)via Western blotting,RT?PCR,methylation?specific PCR(MSP),bisulfite?sequeneing PCR(BSP),respectively, while RNF180 expression in human prostate cancer tissues and paired adjacent non?tumor tissue was detected via immunohistochemistry. Results The expressions of RNF180 mRNA and protein in prostate cancer cells were significantly lower than those in normal prostate cells(P<0.05),op?posite to what was observed for the methylation level of the RNF180 promoter. Additionally,the RNF180 expression in prostate cancer tissue was significantly lower than that in paired adjacent non?tumor tissue. Conclusion The RNF180 promoter is incompletely methylated in prostate can?cer cells,which may be a reason for the decline or silencing of RNF180 expression in cancer cells and tissues.关键词
前列腺癌/RNF180/启动子区甲基化Key words
prostate cancer/RNF180/methylation of promoter分类
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王海光,姜华茂,左知润,龙湟哲,袁观远,贾凡振..RNF180启动子区甲基化在前列腺癌中的作用机制[J].中国医科大学学报,2017,46(6):561-565,5.
