中国医学前沿杂志（电子版）2017，Vol.9Issue(4)：22-26,5.DOI:10.12037/YXQY.2017.04-06
光甘草定对人皮肤成纤维细胞的作用研究
Effects of phytoestrogen Glabridin on human skin fibroblasts in vitro
摘要
Abstract
Objective This study was designed to elucidate the effects of phytoestrogen Glabridin on the proliferation of human skin fibroblast (HSF).Method HSFs were divided into blank control group (only single cell suspension was added), positive control group (treated by 10μmol/L 17β-estradiol) and experience group (0.1, 1, 10 and 100μmol/L Glabridin). After 72 hours, the Cell Counting Kit-8 (CCK-8) assay and 5-ethynyl-2'-deoxyuridine (EdU) incorporation assay were performed to assess cell viability, and the phase of cell generation cycle was determined byflow cytometry.Result The optical density (OD) of 10μmol/L experimental group and positive control group were significantly higher than that of blank control group (P<0.05); OD of 100μmol/L experimental group was significantly lower than that of blank control group (P<0.05), butthere was no significant difference in OD between 10μmol/L experimental group and positive control group (P>0.05). The percentage of EdU labeled positive cells in 10μmol/L experimental group and positive control group were significantly higher than that in blank control group (P<0.05), but there was no significant difference in percentage of EdU labeled positive cells between 10μmol/L experimental group and positive control group (P>0.05). The proportion of S phase in 10μmol/L experimental group and positive control group were significantly higher than that of blank control group (P<0.05), but there was no significant difference in proportion of S phase between 10μmol/L experimental group and positive control group (P>0.05). Conclusion Glabridin promote the proliferation of HSF.关键词
植物雌激素/光甘草定/人皮肤成纤维细胞/细胞增殖Key words
Phytoestrogen/Glabridin/Human skinfibroblast/Cell proliferation引用本文复制引用
王子偲,顾怀宇..光甘草定对人皮肤成纤维细胞的作用研究[J].中国医学前沿杂志（电子版）,2017,9(4):22-26,5.基金项目
广东省教育部科技部产学研结合项目(2012B090600019) （2012B090600019）
