中国肿瘤生物治疗杂志2017，Vol.24Issue(5)：467-471,5.DOI:10.3872/j.issn.1007-385X.2017.05.002
miR-340通过下调CCND1表达增加结直肠癌细胞对5-Fu的耐药
miR-340 enhances chemotherapy resistance of colorectal cancer cells to 5-Fu by down-regulating CCND1 expression
摘要
Abstract
Objective:To investigate the mechanism of coding gene of cell cyclin D1 (CCND1) participating in miR-340-induced inhibition of chemotherapy resistance of colorectal cancer cells to 5-fluorouracil (5-Fu).Methods:Colorectal cancer HCT115 and SW480 cell lines were transfected with si-CCNDland miR340-mimic respectively by transient transfection technology.The sensitivity of cancer cells to 5-Fu after transfection was observed with MTT.The effect of CCND1 on miR-340 mediated suppression of chemotherapy resistance of cancer cells to 5-Fu was validated by double luciferase test.Results:After transient transfection with siCCND1 or over-expression of miR-340,the IC50values of HCT116 and SW480 cell lines were significantly lower than that of control group [CCND1 silencing:10,10 vs 20 μmol/L;miR-340 over-expressing:20,20 vs 40 μmol/L;all P ＜ 0.05).After co-transfection with CCND1 3'UTR wild plasmid and miR-340 inhibitor,the luciferase reporter activity in HCT 116 and SW480 cells were significantly higher than that of empty plasmid transfection group or mimic transfection group (P ＜ 0.01).Conclusion:CCND1,as an unfavorable factor,enhances the chemo-resistance of colorectal cancer cells to 5-FU by inhibiting the expression of miR-340.关键词
细胞周期蛋白D1/CCND1基因/miR-340/结直肠癌/5-氟尿嘧啶/化疗耐药Key words
cyclin D1/CCND1 gene/miR-340/colorectal cancer/5-fluorouracil (5-Fu)/chemotherapy resistance分类
医药卫生引用本文复制引用
姜靖雯,陈学武,方唯意,罗荣城..miR-340通过下调CCND1表达增加结直肠癌细胞对5-Fu的耐药[J].中国肿瘤生物治疗杂志,2017,24(5):467-471,5.基金项目
广东省自然科学基金资助项目(No.2015 A030311005).Project supported by the Natural Science Foundation of Guangdong Province,China(No.2015A030311005) （No.2015 A030311005）
