中国肿瘤生物治疗杂志2017，Vol.24Issue(5)：484-489,6.DOI:10.3872/j.issn.1007-385X.2017.05.005
沉默CHD1L对前列腺癌PC3细胞恶性生物学行为的影响及其可能机制
Effect of CHD1L gene silencing on malignant biologic behaviors of prostate cancer PC3 cell line and its possible mechanism
摘要
Abstract
Objective:To explore the influence of CHD1L gene (chromodomain helicase/ATPase DNA binding protein l-like gene) on the invasion and migration ability of prostate cancer cells and the potential mechanisms.Methods:Realtime fluorescent quantitative-PCR assay was carried out to detect mRNA expression of CHD1L gene in prostate cancer cell lines LNCAP,PC3 and DU 145 as well as normal prostate epithelial cell line RWPE-1.siRNA transfection was used to deplete CHD1L expression in PC3 cells.Then Transwell invasion assay and wound healing test were employed to detect the effect of CHD1L silencing on the invasion and migration ability of PC3 cells.Protein expressions of MMP-9,N-cadherin and E-cadherin were examined by Western blotting assay.Results:Compared to normal prostate epithelial cells,three prostate cancer cell lines all showed significantly higher expression of CHD1L mRNA (P ＜ 0.01),and PC3 cells demonstrated the highest CHD1L mRNA expression.Invasion assay showed that the trans-membrane cell number in CHD1L silencing group was significantly lower than that in negative and blank control groups (49.67 ± 6.67 vs 113.67 ± 5.69 and 112.00 ± 12.49,P ＜ 0.05).Wound healing test also exhibited lower healing degree in CHD1L silencing group than that in negative and blank control groups at48 h ([21.27 ±3.27]％ vs [48.47 ±5.72]％ and [49.93 ±3.35]％,respectively,P ＜ 0.05).Furthermore,protein expressions of MMP-9 and N-cadherin decreased while E-cadherin increased in CHD1L gene depletion cells.Conclusions:CHD1L silencing could dampen the invasion and migration ability of prostate cancer PC3 cell line,and this might be accomplished by regulating MMP-9 and EMT related proteins.关键词
前列腺癌/染色质解旋酶DNA结合蛋白1样基因/侵袭/迁移Key words
prostate cancer/chromodomain helicase/ATPase DNA binding protein 1-like (CHD1L) gene/invasion/migration分类
医药卫生引用本文复制引用
崔发财,陈瑜,秦望森..沉默CHD1L对前列腺癌PC3细胞恶性生物学行为的影响及其可能机制[J].中国肿瘤生物治疗杂志,2017,24(5):484-489,6.基金项目
河南省医学科技攻关计划项目(No.201303155).Project supported by the Key Task Project in Medical Science Research Program of Henan Province(No.201303155) （No.201303155）
