中国肿瘤生物治疗杂志2017，Vol.24Issue(5)：490-496,7.DOI:10.3872/j.issn.1007-385X.2017.05.006
EZH2和H3K27me3的表达对食管癌细胞迁移和侵袭能力的影响
Effects of EZH2 and H3K27me3 expression on migration and invasion of esophageal carcinoma cells
摘要
Abstract
Objective:To study the effect of enhancer of zeste homolog 2 (EZH2) and histone H3 methylated Lys27 (H3K27me3) on the migration and invasion ability of esophageal squamous cell cancer (ESCC) cells by transfection of vectors expressing/knock down EZH2.Methods:Quantificational real-time PCR (qRT-PCR) and Western blotting were performed to evaluate the expression levels of EZH2 in esophageal carcinoma cell lines (KYSE30,KYSE170,TE1,Eca109),as well as the effect of over-expression or knockdown of EZH2 on H3K27me3 expression in ESCC cell lines.Effect of EZH2 over-expression or knockdown on migration and invasion abilities of ESCC cells were examined by wound healing assay and Transwell invasion test,respectively.The expression level of MMPs in ESCC cells after over-expression or knockdown of EZH2 were detected by qRT-PCR.Results:The expressions of EZH2 and H3K27me3 in Eca109 and TE1 cells were significantly higher than those in KYSE30 and KYSE170 cells (P ＜ 0.05).Over-expression of EZH2 promoted the expression of H3K27me3 in KYSE30 and KYSE170 cell lines (P ＜ 0.05).Knockdown of EZH2 inhibited the expression of H3K27me3 in Eca109 and TE1 cell lines (P ＜ 0.05).The trans-membrane number of KYSE30 and KYSE170 cells was significantly increased ([281.33 ± 4.10] vs [132.00 ± 4.00],P ＜ 0.05;[241.67 ± 4.04] vs [105.33 ±3.51],P ＜ 0.05),and the migration distance of KYSE30 and KYSE170 cells was significantly increased ([63.6 ± 1.2] vs [23.0 ± 2.3] μm,P ＜ 0.05;[62.5 ± 2.5] vs [21.2 ± 1.0] μm,P ＜ 0.05) after over-expression of EZH2.Knockdown of EZH2 significantly decreased the trans-membrane number of Eca109 and TE1 cells (all P ＜0.05),and the migration distance was also decreased after transfection with shEZH2 (all P ＜ 0.05).Conclusion:EZH2 can enhance trimethylation of lysine 27 on histone H3 (H3K27) of the target gene promoters,and promote the migration and invasion abilities of esophageal carcinoma cell lines.关键词
Zeste同源物增强子2/Lys27位点三甲基化组蛋白H3/食管鳞状细胞癌/基质金属蛋白酶/迁移/侵袭Key words
enhancer of zeste homolog 2 (EZH2)/histone H3 methylated Lys27 (H3K27me3)/esophageal squamous cell cancer (ESCC)/matrix metalloproteinases (MMPs)/migration/invasion分类
医药卫生引用本文复制引用
刘飞,尹丹静,黄卫娜,谷丽娜,吴云艳,刘世娜,桑梅香..EZH2和H3K27me3的表达对食管癌细胞迁移和侵袭能力的影响[J].中国肿瘤生物治疗杂志,2017,24(5):490-496,7.基金项目
河北省科技支撑计划资助项目(No.14277732D,No.152777184).Project supported by the Science and Technology Supporting Program of Hebei Province(No.14277732D,No.152777184) （No.14277732D,No.152777184）
