中国肿瘤生物治疗杂志2017，Vol.24Issue(5)：503-509,7.DOI:10.3872/j.issn.1007-385X.2017.05.008
人胎盘提取物抑制结肠癌细胞的增殖及迁移
Human placenta extracts suppresses the proliferation and migration of human colon cancer cells
摘要
Abstract
Objective:To explore the effect of human placenta extracts (HPE) on the proliferation and migration of human colon cancer cell lines (Caco-2 and SW480).Methods:Human colon cancer Caco-2 and SW480 cell lines were treated with different concentrations of HPE.Cell proliferation was determined by CCK-8,Ki-67 and CFSE dilution.The effect of HPE on cell apoptosis and cell cycle were detected by Annexin-V/PI and DAPI staining.The expressions of apoptosis related gene BAX,CDK2 and CyclinA2 were determined by Real-time PCR.The migration efficiency of cell was measured by wound-healing assay.Human colon cancer cells were subcutaneously injected into the nude mice to establish xenograft model,and the mice were randomly divided into different groups and given different treatments of HPE and/or 5-fluorouracil (5-Fu).The tumor volume and body weight were recorded.Results:The cell proliferation ability of both Caco-2 and SW480 cells cultured with HPE was lower than that of control group (0.82 ± 0.01 vs 0.96 ± 0.02,P ＜0.01;0.90 ± 0.03 vs 0.96 ± 0.02,P ＜ 0.05);and the apoptotic rates in HPE treatment group were significantly higher than control group ([20.47 ± 1.32]％ vs [11.01 ±3.82]％,P＜0.01;[20.70 ±5.19]％ vs [8.00 ±2.69]％,P＜0.05),accompanied with cytoplasmic reduction,nuclear pyknosis and increased BAX gene expression (3.23 ± 1.90 vs 1.00 ± 0.00;2.25 ± 0.55 vs 1.00 ± 0.00,all P ＜ 0.05).Furthermore,S-phase arrest with an apoptosis peak was observed in HPE treatment group,and decreased mRNA expressions of CDK2 and CyclinA2 (all P ＜ 0.05) in Caco-2 cells were also observed.Moreover,an obvious lower wound-healing rate was identified in cells treated with HPE ([0.17 ±0.29] vs [1.50 ± 0.50] mm,P ＜ 0.05).The tumor volume of mice in HPE-treated group was smaller than that of control group,and higher survival rate was observed than that of 5-Fu alone group.Conclusion:This study indicated that HPE could inhibit the proliferation and migration ability of human colon cancer cells both in vitro and in vivo,partly through enhancing cell apoptosis and S-phase arrest,which may contribute to the inhibition on growth of certain tumor cells.关键词
结肠癌/人胎盘提取物/Caco-2细胞/SW480细胞/细胞凋亡/细胞周期Key words
colon cancer/human placenta extracts(HPE)/Caco-2 cell/SW480 cell/apoptosis/cell cycle分类
医药卫生引用本文复制引用
李静,周海燕,周萍萍,雷慧群,颜海健,余芳..人胎盘提取物抑制结肠癌细胞的增殖及迁移[J].中国肿瘤生物治疗杂志,2017,24(5):503-509,7.基金项目
国家自然科学基金资助项目(No.81560266) （No.81560266）
贵州医科大学附属医院博士启动基金资助项目.Project supported by the National Natural Science Foundation of China(No.81560266),and the Doctor Start-up Foundation of Hospital Affiliated to Guizhou Medical University （No.81560266）
