中国肿瘤生物治疗杂志2017，Vol.24Issue(5)：510-515,6.DOI:10.3872/j.issn.1007-385X.2017.05.009
rNDV IL-29对肺腺癌A549细胞恶性生物学行为的影响
Effect of rNDV IL-29 on malignant biological behavior of lung adenocarcinoma A549 cells
摘要
Abstract
Objective:To investigate the effects of recombinant Newcastle disease virus (rNDV) expressing IL-29 (rNDV IL-29)on the proliferation,migration and apoptosis of lung adenocarcinoma A549 cells.Methods:A549 cells were treated with rNDV IL-29,NDV and PBS respectively,namely rNDV IL-29 group,NDV group and control group.The protein and gene expression of IL-29 in A549 cells were examined by RT-PCR,immunofluorescence and Western blotting;MTT assay,colony formation assay and scratch assay were used to evaluate the effect of rNDV IL-29 on proliferation and migration ability of A549 cells;Flow cytometry and Western blotting were used to examine the effect of rNDV IL-29 on apoptosis of A549 cells.Results:Compared with NDV group and control group,(1) IL-29 protein and gene expressions were dramatically increased in rNDV IL-29 group.(2) the rate of cell proliferation in rNDV IL-29 group was significantly inhibited ([56.48 ± 11.89]％ vs [42.18 ±6.18]％,P ＜0.05),and migration ability of A549 cells in rNDV IL-29 group was statistically decreased ([5.00 ±4.00]％ vs [32.43 ±3.71]％,[84.57 ±3.96]％,all P＜0.05).(3) the expression of caspase3 in rNDV IL-29 group was significantly up-regulated ([29.67 ± 1.53] ％ vs [18.33 ± 3.06] ％,[7.0 ± 6.08] ％,all P ＜ 0.05),while Bcl-2/bax level was remarkably reduced ([12.00 ± 2.65] ％ vs [42.33 ±5.86] ％,[67.00 ± 6.25] ％,all P ＜ 0.05].Conclusion:rNDV IL-29 inhibits the proliferation and migration of A549 cells and promotes apoptosis of A549,suggesting that rNDV IL-29 may be served as a potential anticancer drug.关键词
重组新城疫病毒/白细胞介素-29/肺腺癌/凋亡Key words
recombinant Newcastle disease virus/IL-29/adenocarcinoma of lung/apoptosis分类
医药卫生引用本文复制引用
严玉兰,刘沙,李米,赵英海,金惠,尹超云,步雪峰..rNDV IL-29对肺腺癌A549细胞恶性生物学行为的影响[J].中国肿瘤生物治疗杂志,2017,24(5):510-515,6.基金项目
江苏省自然科学基金资助项目(No.BK20151333).Project supported by the Natural Science Foundation of Jiangsu Province (No.BK20151333) （No.BK20151333）
