中国肿瘤生物治疗杂志2017，Vol.24Issue(5)：527-532,6.DOI:10.3872/j.issn.1007-385X.2017.05.012
多因子固相抗体芯片筛选评价免疫细胞治疗肺癌的生物标志物
Biomarkers of lung carcinoma for evalution of the immunocytotherapy selected by solid phase antibody chip against multiple factors
摘要
Abstract
Objective:Applying solid phase antibody chip against multiple factors to screen serum marker proteins of the patients with lung carcinoma who were treated by immunocytotherapy as clinical diagnosis index and to establish an evaluation model for clinical efficacy of the patients with lung carcinoma.Methods:Using Proteome ProfilerTM solid phase antibody chip against multiple factors and Image J software to analyze gray scale/optical density of the serum marker proteins from 5 patients with advanced lung carcinoma,5 patients with improved lung carcinoma and 10 healthy individuals.The protein gray values obtained were analyzed by SPSS software,and Fisher model for evaluation of clinical efficacy of the patients with lung carcinoma was established.Results:Analyzing and comparing the common marker proteins among the patients with advanced lung carcinoma,the patients with improved lung carcinoma and the healthy individuals,8 proteins that are dipeptidyl peptidase Ⅳ,growth hormone,IL-4,myeloperoxidase,osteopontin,receptor of advanced glycation endproducts,TNF-o,and urokinase type plasminogen activator receptor with significant difference (P ＜ 0.05) were obtained.Clustering and analyzing data for all of the patients and the healthy individuals in the experiment found that the 8 proteins can very well distinguish the patients with advanced lung carcinoma from the patients with improved lung carcinoma and the healthy individuals.The Fisher model for lung carcinoma was confirmed.Conclusion:The solid phase antibody chip against multiple factors and optimal statistical methods could screen out the serum protein biomarkers related to occurrence,development and efficacy of lung carcinoma,which could establish a certain basis of clinical trail for researches on the mechanism of lung carcinogenesis as well as clinical diagnosis and treatment of the lung carcinoma.It could play an important guiding role in the individualized treatment of the lung carcinoma.关键词
肺癌/免疫细胞治疗/生物标志物/统计学方法Key words
lung carcinoma/immunocytotherapy/biomarker/statistic method分类
医药卫生引用本文复制引用
梁露花,初廷广,李东霖,王思亮,李文欣,项荣武..多因子固相抗体芯片筛选评价免疫细胞治疗肺癌的生物标志物[J].中国肿瘤生物治疗杂志,2017,24(5):527-532,6.基金项目
辽宁省教育厅基金资助项目(No.201610163L28).Project supported by the Foundation of Liaoning Education Department (No.201610163L28) （No.201610163L28）
