中国卒中杂志2017，Vol.12Issue(6)：489-495,7.DOI:10.3969/j.issn.1673-5765.2017.06.005
门冬氨酸钾减轻大鼠可控性皮质打击伤引起的脑损伤
Potassium Aspartate Alleviates Brain Injury Induced by Controlled Cortical Impact in Rats
摘要
Abstract
Objective Potassium aspartate (PA), as an electrolyte supplement, is widely used in clinical practice. Previous study found PA had neuroprotective effects against apoptosis after cerebral ischemia/reperfusion in rats. This study is to investigate whether PA has protective effects on traumatic brain injury. Methods Traumatic brain injury (TBI) was induced by controlled cortical impact (CCI) in rats. Vehicle treatment group or potassium aspartate treatment group were administered saline via intraperitoneal injection or PA at the dose of 62.5 mg/kg at 30 minutes after onset of CCI. The brain blood perfusion, modified Neurological Severity Score (mNSS), and cortical lesion volume were observed. Brain edema brain ATP contents, lactic acid concentration, and Na+/K+-ATPase activities were measured. Results In controlled cortical impact induced cortical injury in rats, compared with vehicle-only treatment, PA treatment during the acute phase of MCAO at the dose of 62.5 mg/kg significantly improved neurological deficits (P<0.05), decreased the cortical lesion volume (P<0.01), and alleviated brain edema (P<0.05). In addition, PA treatment significantly reduced the loss of ATP (P<0.01),reduced lactic acid levels (P<0.05), and increased the activity of Na+/K+-ATPase (P<0.05). Conclusion PA had neuroprotective effects on TBI through increasing ATP, Na+/K+-ATPase activity, and reducing brain edema. It provides experimental evidence for the clinical application of PA in clinical treatment of brain injury.关键词
门冬氨酸钾/脑损伤/可控性皮质打击伤/钠钾ATP酶Key words
Aspartic acid/Brain injury/Controlled cortical impact/Ischemia/Na+/K+-ATPase引用本文复制引用
顾漪,赵育梅,苏玉金..门冬氨酸钾减轻大鼠可控性皮质打击伤引起的脑损伤[J].中国卒中杂志,2017,12(6):489-495,7.基金项目
辽宁药联制药有限公司"重大新药创制"科技重大专项"十二五"计划课题(2012ZX09401004) （2012ZX09401004）
