中南大学学报（自然科学版）2017，Vol.48Issue(5)：1134-1140,7.DOI:10.11817/j.issn.1672-7207.2017.05.002
磁性富集荧光法检测大肠杆菌
Fluorescent detection of Escherichia coli based on magnetic enrichment
摘要
Abstract
A novel sensitive assay was investigated for the highly sensitive and rapid detection of Escherichia coli O157:H7. It was based on magnetic Fe3O4/graphene oxide (MGO) and DNA probes which was modified by 4-N-aminoethyl-N- hydroxyethyl-1,8-naphthalimide (AHA). First, the ssDNA labelled with AHA was employed as a capture probe and adsorbed on the surface of MGO. When the target ssDNA appeared, capture probe hybridized with it in part. Then target ssDNA was fished and enriched via external magnetization. Afterwards, a solution of release probe was added to the magnetic particles to complete the hybridization which resulted in separating the AHA from MGO. The results show that the linear relationship is found between logarithm value of E.coli and the ratio of fluorescence intensities (F/F0) from 150 to 1.5×106 /mL. The detection limit is 100 cfu/mL. The highly sensitive, rapid and simple means is demonstrated to be a noble tool for the detection of pathogenic bacteria.关键词
大肠杆菌O157:H7/磁性氧化石墨烯/富集/DNA杂交/萘酰亚胺Key words
Escherichia coliO157:H7/magnetic Fe3O4/graphene oxide/enriching/DNA hybridization/4-N-aminoethyl- N-hydroxyethyl-1/8-naphthalimide分类
资源环境引用本文复制引用
杨宇,牛承岗,曾光明..磁性富集荧光法检测大肠杆菌[J].中南大学学报（自然科学版）,2017,48(5):1134-1140,7.基金项目
国家自然科学基金资助项目(51541801) (Project(51541801) supported by supported by the National Natural Science Foundation of China) （51541801）
