质谱学报2017，Vol.38Issue(3)：282-293,12.DOI:10.7538/zpxb.youxian.2016.0065
基于UPLC-Q-TOF MS的吴茱萸致肝毒性部位及入血成分分析
Analysis of Euodia rutaecarpa Hepatotoxic Part and Absorbed Components in Rat Serum Based on UPLC-Q-TOF MS
摘要
Abstract
A method of ultra performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry (UPLC-Q-TOF MS) was established to analyze the chemicals in Euodia rutaecarpa hepatotoxic part and metabolic components in rat blood.Under the optimized condition of chromatography and mass spectrometry, the data collection for the compounds in the mass range of m/z 50-1 200 was performed using positive ion scanning mode within 12 min.The collected data were analyzed based on UNIFI software combined with artificial analysis.According to the precise mass information of precursor ions and fragment ions offered by high resolution mass spectrometry, the molecule composition of compound was preliminarily speculated.Finally, a total of 29 constituents in hepatotoxic part are identified combined with substances and reference data, including 9 indole alkaloids, 10 quinolone alkaloids, 5 triterpenoids, 3 flavonoids, one other alkaloid and one organic acid.21 compounds in blood are characterized, including 15 prototypes and 6 metabolites (a compound unknown) by comparing the base peak ion chromatograms of samples in vivo and in vitro combined with MS data, compound fragmentation laws and published literatures.The metabolic pathways of metabolites were further speculated.This method can provide, a reliable data support to indicate hepatotoxic components of Euodia rutaecarpa.关键词
吴茱萸/肝毒性部位/成分分析/代谢产物/超高效液相色谱-四极杆-飞行时间质谱(UPLC-Q-TOFMS)Key words
Euodia rutaecarpa/hepatotoxic part/constituent analysis/metabolites/ultra performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry (UPLC-Q-TOF MS)分类
化学化工引用本文复制引用
李文兰,孙向明,陈晨,刘悦,宋辉,丁晶鑫,徐蓓蕾,阎新佳..基于UPLC-Q-TOF MS的吴茱萸致肝毒性部位及入血成分分析[J].质谱学报,2017,38(3):282-293,12.基金项目
哈尔滨市科技局科技创新人才项目(2015RQXXJ011) （2015RQXXJ011）
黑龙江省教育厅科研项目(12541192)资助 （12541192）
