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紫丁香蘑粗多糖的体外抗氧化活性研究

李男男 李环明 薛春梅 赵永勋 李佳琳 杜春梅 李丽 靖桂云

安徽农业科学2017,Vol.45Issue(14):63-65,3.
安徽农业科学2017,Vol.45Issue(14):63-65,3.

紫丁香蘑粗多糖的体外抗氧化活性研究

The Antioxidant Activity of Crude Polysaccharide in Lepista nuda

李男男 1李环明 1薛春梅 1赵永勋 2李佳琳 1杜春梅 1李丽 1靖桂云2

作者信息

  • 1. 佳木斯大学生命科学学院,黑龙江佳木斯 154007
  • 2. 佳木斯大学应用微生物研究所,黑龙江佳木斯 154007
  • 折叠

摘要

Abstract

[Objective]To study the antioxidant activity of crude polysaccharide in Lepista nuda mycelium.[Method]The mycelium polysaccharide of Lepista nuda was extracted by microwave-assisted method.The crude polysaccharide of fermentation broth was extracted by solid-liquid separation and centrifugation.The change in the capacity of crude polysaccharide scavenges free radical with the methods of DPPH,salicylic acid colorimetry and three phenol autoxidation was determined.[Result]The crude polysaccharide of mycelium and fermentation broth both had stronger antioxidant ability.However,the antioxidant capacity of two kinds of crude polysaccharide was different;The ability of scavenging free radical of polysaccharide from mycelium was stronger than that of polysaccharide in fermentation broth.In a certain range of concentration,if the concentration of polysaccharide increased its antioxidant capacity also increased,which was dose-dependent.[Conclusion]The crude polysaccharide of mycelium and fermentation broth both had stronger antioxidant ability,and the former was stronger than the latter.

关键词

紫丁香蘑/粗多糖/抗氧化活性/自由基

Key words

Lepista nuda/Crude polysaccharide/Antioxidant activity/Free radical

分类

农业科技

引用本文复制引用

李男男,李环明,薛春梅,赵永勋,李佳琳,杜春梅,李丽,靖桂云..紫丁香蘑粗多糖的体外抗氧化活性研究[J].安徽农业科学,2017,45(14):63-65,3.

基金项目

黑龙江省教育厅科学技术面上项目(12531696) (12531696)

佳木斯大学科技青年基金项目(Sq2013-029). (Sq2013-029)

安徽农业科学

0517-6611

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