安徽农业科学2017,Vol.45Issue(14):63-65,3.
紫丁香蘑粗多糖的体外抗氧化活性研究
The Antioxidant Activity of Crude Polysaccharide in Lepista nuda
摘要
Abstract
[Objective]To study the antioxidant activity of crude polysaccharide in Lepista nuda mycelium.[Method]The mycelium polysaccharide of Lepista nuda was extracted by microwave-assisted method.The crude polysaccharide of fermentation broth was extracted by solid-liquid separation and centrifugation.The change in the capacity of crude polysaccharide scavenges free radical with the methods of DPPH,salicylic acid colorimetry and three phenol autoxidation was determined.[Result]The crude polysaccharide of mycelium and fermentation broth both had stronger antioxidant ability.However,the antioxidant capacity of two kinds of crude polysaccharide was different;The ability of scavenging free radical of polysaccharide from mycelium was stronger than that of polysaccharide in fermentation broth.In a certain range of concentration,if the concentration of polysaccharide increased its antioxidant capacity also increased,which was dose-dependent.[Conclusion]The crude polysaccharide of mycelium and fermentation broth both had stronger antioxidant ability,and the former was stronger than the latter.关键词
紫丁香蘑/粗多糖/抗氧化活性/自由基Key words
Lepista nuda/Crude polysaccharide/Antioxidant activity/Free radical分类
农业科技引用本文复制引用
李男男,李环明,薛春梅,赵永勋,李佳琳,杜春梅,李丽,靖桂云..紫丁香蘑粗多糖的体外抗氧化活性研究[J].安徽农业科学,2017,45(14):63-65,3.基金项目
黑龙江省教育厅科学技术面上项目(12531696) (12531696)
佳木斯大学科技青年基金项目(Sq2013-029). (Sq2013-029)