安徽农业科学2017，Vol.45Issue(14)：77-79,3.
β-胡萝卜素发酵促进因子筛选及均匀试验设计优化
Screening of Beta Carotene Fermentation Accelerant and Optimization of Uniform Experimental Design
摘要
Abstract
[Objective] In order to improve the fermentative production of β-carotene by Blakeslea trispora.[Method] The dosage concentration and interactions of 6 fermentation accelerants were studied.Firstly,using single factor experiments to study the effective concentration range of fermentation accelerants.Then on this basis,by using uniform experiment to optimize the fermentation culture.[Result] The optimal fermentation accelerant formula was composed of 0.4 mmol/L MgCl2,20.0 μmol/L H2O2,0.1% Span 20,1.8% n-decane,0.2 mmol/L sodium formate and 1.1 g/L trisodium citrate.The β-carotene production was up to 2.27 g/L,77.30% higher than that of control group.[Conclusion] The optimized fermentation accelerants could significantly improve the β-carotene production.关键词
β-胡萝卜素/发酵促进剂/单因素试验/均匀试验设计Key words
β-carotene/Fermentation accelerant/Single factor experiment/Uniform experimental design分类
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杨晔,尹金凤,李泓全..β-胡萝卜素发酵促进因子筛选及均匀试验设计优化[J].安徽农业科学,2017,45(14):77-79,3.
