安徽农业科学2017，Vol.45Issue(14)：103-106,4.
濒危药材金线莲愈伤组织诱导及分化成苗研究
Study on Callus Induction and Seeding Differentiation of the Rare Medicinal Species Anoectochilus roxburghii
摘要
Abstract
[Objective] The research aimed to establish an effective recycling system for Anoectochilus roxburghii, and lay the technical foundation for its industrial production.[Method] As explant of leaf, stem, stem segment with node and adventitious bud of Anoectochilus roxburghii, the aseptic system was established, and then the induction rate of callus was used as an index to optimize the type and concentration of hormones in the culture medium of different explants (callus induction and differentiation) by orthogonal design.[Result]In the test of Anoectochilus roxburghii, four explants, the callus induction rate of adventitious bud was the highest, up to 96.67%, the optimum medium formula was MS+0.5 mg/L NAA+4.0 mg/L BA+0.3 mg/L KT.On the basis of the medium, the callus was subcultured on the differentiation medium(MS+0.5 mg/L NAA+2.0 mg/L BA) and the proliferation medium (MS+0.2 mg/L NAA+2.0 mg/L BA) to form a rootless test tube.[Conclusion]Asepsis reproductive system has been established, which solves the bottleneck problem that the predecessors pointed out that the callus induction rate of Anoectochilus roxburghii was very low,the efficiency of tissue culture seedlings have greatly improved.关键词
金线莲/不定芽/愈伤组织/分化成苗Key words
Anoectochilus roxburghii/Adventitious buds/Callus/Seeding differentiation分类
农业科技引用本文复制引用
许晴,穆梁丽,冀爱青..濒危药材金线莲愈伤组织诱导及分化成苗研究[J].安徽农业科学,2017,45(14):103-106,4.基金项目
国家大学生创新项目(201510121003). （201510121003）
