安徽农业科学2017，Vol.45Issue(14)：132-133,140,3.
致病性副溶血性弧菌特异性三重PCR检测方法研究
Study on the Specific Triplex-PCR Method of Pathogenic Vibrio parahaemolyticus
摘要
Abstract
[Objective] To establish a rapid specific triplex-PCR method of pathogenic Vibrio parahaemolyticus by detecting gyrase, tdh and toxR genes simultaneously.[Method] The genomic DNA of V.parahaemolyticus ATCC33847 was amplified by three pairs of specific primers individually, the optimum amplification condition for each pair of primers was determined.Through optimizing the concentration and ratio of primers and anneal temperature, a triplex-PCR method which can simultaneously amplify three target genes was established.[Result] The bands exhibiting the sequences of 91, 269 and 368 bp were amplified by gyrase,tdh,toxR respectively in an optimum triplex-PCR reaction.[Conclusion] The triplex-PCR method provides a new technical mean for the rapid detection of pathogenic V.parahaemolyticus.关键词
致病性副溶血性弧菌/三重PCR/gyrase基因/tdh基因/toxR基因Key words
Pathogenic Vibrio parahaemolyticus/Triplex-PCR/gyrase gene/tdh gene/toxR gene分类
轻工纺织引用本文复制引用
金雷,陈瑜,张小军,施慧..致病性副溶血性弧菌特异性三重PCR检测方法研究[J].安徽农业科学,2017,45(14):132-133,140,3.基金项目
浙江省分析测试科技计划项目(2015C37065) （2015C37065）
浙江省自然科学基金项目(Y15C190018). （Y15C190018）
