安徽农业科学2017，Vol.45Issue(15)：26-27,36,3.
番茄灰霉病的拮抗菌筛选及分子鉴定
Screening and Identification of Antagonistic Bacteria from Tomato Botrytis cinerea
摘要
Abstract
[Objective] To screen antagonistic bacteria from tomato Botrytis cinerea, and identification was conducted by molecular biology methods.[Method] With tomato Botrytis cinerea as test bacteria, antagonistic bacteria was screened from the farming soil.The method of plate confrontation was used to screen antagonistic bacteria, antagonistic bacteria was identifed through 16S rDNA sequence analysis.[Result] 13 strains exhibiting antagonistic activities against Botrytis cinerea were screened from rhizosphere soil of tomato.Test on antagonistic ability revealed that 3 strains showed strong and steady antagonism against B.cinerea, the inhibition rate was above 60.00%.Morphological observation and 16S rDNA sequence analysis showed that the three strains were Paenibacillus jamilae, Paenibacillus polymyxa and Bacillus subtilis.[Conclusion]The study provides theoretical basis for the biological control of B.cinerea.关键词
番茄灰霉病/拮抗菌/筛选/鉴定Key words
Tomato Botrytis cinerea/Antagonistic bacteria/Screening/Identification分类
农业科技引用本文复制引用
刘程程,明亮,储西平,陈怀谷..番茄灰霉病的拮抗菌筛选及分子鉴定[J].安徽农业科学,2017,45(15):26-27,36,3.基金项目
江苏省农业科技自主创新资金项目(Cx〔15〕1029). （Cx〔15〕1029）
