安徽农业科学2017，Vol.45Issue(15)：51-53,3.
微囊藻毒素3种异构体的提取净化研究
Study on the Extraction and Purification of 3 Isomers of Microcystins
摘要
Abstract
[Objective]To establish method for extracting and purifying microcystins.[Method]The effects of solid phase extraction column, eluent on Extraction and purification of 3 isomers of microcystins (MCs, LR, YR and RR) were analyzed and compared.A method for extraction and purification of MCs by solid phase extraction was established.[Result]The AccuBOND II ODS C18 solid phase extraction column has a high recovery rate for 3 isomers in MCs, and the recovery rate was above 80%.The lower the methanol concentration was, the worse the leaching capacity of the eluent, and 10% methanol leaching can remove most of the pigment impurities, and the loss rate of the 3 isomers was smaller.The higher the methanol concentration was, the stronger the elution capacity was, and the lower concentration of methanol eluent was 70% and 80%.There was less impurity in the eluent collection liquid, and 80% of the methanol was an ideal eluent.[Conclusion]The method of extraction and purification of Microcystis was simple and feasible, which can provide scientific basis for the industrialization application in the future.关键词
微囊藻毒素/固相萃取/提取/净化/回收率Key words
Microcystins/Solid phase extraction/Extraction/Purification/Recovery分类
资源环境引用本文复制引用
李莉,蔡金傍,赵志强,孙旭..微囊藻毒素3种异构体的提取净化研究[J].安徽农业科学,2017,45(15):51-53,3.基金项目
"十二五"村镇建设领域国家科技计划课题(2014BAL 02B04). （2014BAL 02B04）
