安徽农业科学2017，Vol.45Issue(15)：57-59,84,4.
水环境中诺如病毒RT-LAMP快速检测方法的建立及应用
Establishment and Application of RT-LAMP Rapid Detection Method for Norwalk Virus in Water Environment
摘要
Abstract
[Objective] Based on norovirus (NV) gene conserved region, to design a RT-LAMP degenerate primer, and to establish a simple, specific and sensitive method for the determination of NV in water environment samples by RT-LAMP.[Method]Through experiments, the reaction system was established by optimizing reaction temperature (64 ℃), reaction time (40 min) and Mg2+ concentration (2 mmol/L).[Result]The method only needed a water bath to complete the test in an hour, and could be observed directly with the naked eye, and the sensitivity was 100 times higher than that of the ordinary RT-PCR.The design of degenerate primers enhanced specificity and could detect multiple serotypes of NV.[Conclusion]In the sample detection, the sensitivity of RT-LAMP technology was 97.7%, higher than 90.7% of RT-PCR.It was more suitable for rapid detection of field samples and crude Laboratory.关键词
诺如病毒/逆转录/环介导等温扩增Key words
Norwalk virus/Reverse transcription/Loop-mediated isothermal amplification分类
资源环境引用本文复制引用
罗鸿斌..水环境中诺如病毒RT-LAMP快速检测方法的建立及应用[J].安徽农业科学,2017,45(15):57-59,84,4.基金项目
广东省公益研究与能力建设专项资金项目(2014A020212692) （2014A020212692）
东莞市高等院校、科研机构科技计划一般项目(2014106101006) （2014106101006）
广东高校省级重点平台和重大科研项目(2016GXJK156). （2016GXJK156）
