安徽农业科学2017，Vol.45Issue(15)：95-99,5.
液相色谱串联质谱法测定动物组织中17种β-兴奋剂残留
Determination of 17 β-agonists Residual in Animal Tissue by Liquid Chromatography-Tandem Mass Spectrometer
招钰娟 1邓晓华 1甘沛明 1梁雪琪 1黎小鹏2
作者信息
- 1. 中山市农产品质量监督检验所,广东中山 528400
- 2. 中山市三乡镇农产品检验检测站,广东中山 528463
- 折叠
摘要
Abstract
[Objective] A sensitive and rapid HPLC-MS/MS method was developed for the determination of Beta-adrenergic agonistsresidues in animal tissue.[Method] Samples were subjected to extraction, followed by solid phase extraction clean-up before HPLC-MS/MS analysis.The pretreatment conditions and mass spectra parameters were studied.[Result] The developed standard curves of 17 Beta-adrenergic agonists all displayed good linearity over the concentration range of 0.5-50.0 ng/mL, and coefficient correlation (R2) between 0.999 88-0.995 70.Mean recovery rates of 6 replicates for Beta-adrenergic agonist in a negative pork sample spiked at two levels between 63.20%-109.92%;62.99%-112.19%, RSD was 0.63%-11.50% and 0.33%-8.93%.[Conclusion] This method proved to be of stability, high sensitivity and good selectivity and provided satisfying determination results.Therefore, its application deserves to be popularized among various food testing laboratories.关键词
β-兴奋剂/动物组织/液相色谱串联质谱/残留Key words
β-agonists/Animal tissue/Liquid chromatography-tandem mass spectrometer/Residual分类
农业科技引用本文复制引用
招钰娟,邓晓华,甘沛明,梁雪琪,黎小鹏..液相色谱串联质谱法测定动物组织中17种β-兴奋剂残留[J].安徽农业科学,2017,45(15):95-99,5.
