安徽中医药大学学报2017，Vol.36Issue(3)：64-68,5.DOI:10.3969/j.issn.2095-7246.2017.03.019
鱼腥草素钠诱导临床耐药白假丝酵母菌生物膜细胞凋亡
Sodium Houttuyfonate Induces Apoptosis of Candida albicans Biofilm Cells with Clinical Drug Resistance
摘要
Abstract
Objective To investigate the effect of sodium houttuyfonate on the apoptosis of Candida albicans biofilm cells with clinical drug-resistance.Methods DAPI staining were performed to observe the morphology of drug-resistant Candida albicans biofilm cell nuclei.FITC-VAD-FMK staining assay was performed to evaluate metacaspase activity in drug-resistant Candida albicans biofilm cells.With the help of JC-1 and DCFH-DA staining, flow cytometry was used to measure mitochondrial membrane potential (MMP) and reactive oxygen species (ROS) level in drug-resistant Candida albicans biofilm cells.Annexin Ⅴ-FITC/PI staining and flow cytometry were used to observe the apoptosis rate of drug-resistant Candida albicans biofilm cells.Results Under the action of sodium houttuyfonate at a certain concentration, drug-resistant Candida albicans biofilm cells had significant increases in ROS level, cell apoptosis rate, and metacaspase activity and a significant reduction in MMP, with fragmentation and shrinkage of cell nucleus.Conclusion Sodium houttuyfonate can induce the apoptosis of drug-resistant Candida albicans biofilm cells.关键词
鱼腥草素钠/耐药白假丝酵母菌/生物膜细胞/凋亡Key words
Sodium houttuyfonate/Drug-resistant Candida albicans/Biofilm cell/Apoptosis分类
医药卫生引用本文复制引用
崔艳艳,施高翔,汪云霞,汪天明,邵菁,汪长中..鱼腥草素钠诱导临床耐药白假丝酵母菌生物膜细胞凋亡[J].安徽中医药大学学报,2017,36(3):64-68,5.基金项目
国家自然科学基金项目(81603167,81573725) （81603167,81573725）
安徽省自然科学基金项目(1508085QH193) （1508085QH193）
